{"refrec":{"BRefID":289294,"RR":"<b>Lozano, J.-C.; Schatt, P.; Botebol, H.; Vergé, V.; Lesuisse, E.; Blain, S.; Carré, I.A.; Bouget, F.-Y.</b> (2014). Efficient gene targeting and removal of foreign DNA by homologous recombination in the picoeukaryote <i>Ostreococcus</i>. <i>Plant J. 78(6)</i>: 1073-1083. <a href=\"https://dx.doi.org/10.1111/tpj.12530\" target=\"_blank\">https://dx.doi.org/10.1111/tpj.12530</a>","BEntID":281331,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Plant J. 78(6)</i>: 1073-1083. <a href=\"https://dx.doi.org/10.1111/tpj.12530\" target=\"_blank\">https://dx.doi.org/10.1111/tpj.12530</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lozano, J.-C.; Schatt, P.; Botebol, H.; Vergé, V.; Lesuisse, E.; Blain, S.; Carré, I.A.; Bouget, F.-Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lozano, J.-C. <i>et al.</i>","Englishabstract":"With fewer than 8000 genes and a minimalist cellular organization, the green picoalga <i>Ostreococcus tauri</i> is one of the simplest photosynthetic eukaryotes. <i>Ostreococcus tauri</i> contains many plant-specific genes but exhibits a very low gene redundancy. The haploid genome is extremely dense with few repeated sequences and rare transposons. Thanks to the implementation of genetic transformation and vectors for inducible overexpression/knockdown this picoeukaryotic alga has emerged in recent years as a model organism for functional genomics analyses and systems biology. Here we report the development of an efficient gene targeting technique which we use to knock out the <i>nitrate reductase</i> and <i>ferritin</i> genes and to knock in a luciferase reporter in frame to the ferritin native protein. Furthermore, we show that the frequency of insertion by homologous recombination is greatly enhanced when the transgene is designed to replace an existing genomic insertion. We propose that a natural mechanism based on homologous recombination may operate to remove inserted DNA sequences from the genome.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Efficient gene targeting and removal of foreign DNA by homologous recombination in the picoeukaryote <i>Ostreococcus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:32:16.007802","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Ostreococcus; homologous recombination; gene targeting; microalgae;genetic transformation; technical advance","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2017-09-21","DateCreate":"2017-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000337600000015","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/tpj.12530"},"refs":null,"anarec":{"AnaID":289294,"PubliDate":2014,"Pagination":"1073-1083","XtraPublOfAnaID":null,"ISBN":null,"Volume":"78","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":175799,"SerRR":"The plant journal. Blackwell Publishing: York.  ISSN 0960-7412; e-ISSN 1365-313X","StandardTitleSer":"The plant journal","ISSN":"0960-7412","AbbrevSer":"Plant J.","StandardTitleMon":null,"StartPage":1073,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lozano","Firstname":"Jean-Claude","Initials":"J.-C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":34471,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Schatt","Firstname":"Philippe","Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":34474,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Botebol","Firstname":"Hugo","Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":252757,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Vergé","Firstname":"Valérie","Initials":"V.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":252764,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lesuisse","Firstname":"Emmanuel","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":252760,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Blain","Firstname":"Stéphane","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":51008,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Carré","Firstname":"Isabelle","Initials":"I.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":270824,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bouget","Firstname":"Francois-Yves","Initials":"F.-Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281331,"AutID":49400,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":307079,"LibID":36,"BRefID":289294,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"307079","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell Publishing","City":"York"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Exploitable Scientific Result","ThestID":169539,"Acronym":"KTPKO","ThesTermPath":"Exploitable Scientific Result"},{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTermPath":"Marine Sciences"},{"ThesaurusTerm":"Marine Genomics","ThestID":169586,"Acronym":"KTPS","ThesTermPath":"Marine Sciences > Marine Genomics"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTermPath":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTermPath":"Scientific Publication"}],"thestermsASFA":[{"ThesaurusTerm":"Exploitable Scientific Result"},{"ThesaurusTerm":"Marine Genomics"},{"ThesaurusTerm":"Marine Sciences"},{"ThesaurusTerm":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":[{"ProID":4198,"Acronym":"ASSEMBLE","ProStandardTitle":"Association of European marine biological laboratories","BYear":2009,"BMonth":3,"EYear":2013,"EMonth":2,"FP7Code":"A-2008","RelID":null,"Relation":null,"DutchTerm":null}],"Physdatasets":null,"spcols":{"990":{"SpName":"Assemble","SpColID":990,"ParSpColID":null,"TopParID":null,"ShortName":"Assemble","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Assemble"},"951":{"SpName":"AssembleMarine","SpColID":951,"ParSpColID":990,"TopParID":990,"ShortName":"AssembleMarine","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":990,"SpColPath":"Assemble/AssembleMarine"},"950":{"SpName":"EMBRC","SpColID":950,"ParSpColID":null,"TopParID":null,"ShortName":"EMBRC","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"EMBRC"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":281331,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":281331,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/tpj.12530","externalID":"10.1111/tpj.12530","URLTypeCode":"DOI","URLID":58169,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Exploitable Scientific Result","ThestID":169539,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"},{"ThesaurusTerm":"Marine Genomics","ThestID":169586,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Exploitable Scientific Result","DutchTerm":null,"ThestID":169539,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"},{"ThesaurusTerm":"Marine Genomics","DutchTerm":null,"ThestID":169586,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Sciences","DutchTerm":null,"ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","DutchTerm":null,"ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","DutchTerm":null,"ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2017-09-18 22:10:53.193000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2017-09-21 08:23:26.587000","timezone_type":3,"timezone":"Europe/Brussels"}}}