{"refrec":{"BRefID":289296,"RR":"<b>Filker, S.; Kaiser, M.; Rosselló-Mora, R.; Dunthorn, M.; Lax, G.; Stoeck, T.</b> (2014). \"<i>Candidatus</i> Haloectosymbiotes riaformosensis\" (Halobacteriaceae), an archaeal ectosymbiont of the hypersaline ciliate <i>Platynematum salinarum</i>. <i>Syst. Appl. Microbiol. 37(4)</i>: 244-251. <a href=\"https://dx.doi.org/10.1016/j.syapm.2014.01.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2014.01.001</a>","BEntID":281333,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Syst. Appl. Microbiol. 37(4)</i>: 244-251. <a href=\"https://dx.doi.org/10.1016/j.syapm.2014.01.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.syapm.2014.01.001</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Filker, S.; Kaiser, M.; Rosselló-Mora, R.; Dunthorn, M.; Lax, G.; Stoeck, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Filker, S. <i>et al.</i>","Englishabstract":"The novel ciliate <i>Platynematum salinarum (Scuticociliatia)</i> was isolated only recently from a thalassohaline solar saltern pond (12%) in Portugal. Scanning electron microscopy showed numerous bacterial-shaped cells covering the complete surface of the ciliate. The rod-shaped epibionts were identified and characterized following the “Full-Cycle rRNA Approach”. The almost full-length 16S rRNA gene sequence was obtained using archaeal-specific primers and two species-specific probes were designed for fluorescence in situ hybridization. The 16S rRNA gene sequence of the epibiotic cells showed 87% sequence identity with the type strain sequence of the closest characterized species <i>Halolamina pelagica</i>. Phylogenetic reconstructions affiliated the novel organism to the genus <i>Halolamina</i> (Halobacteria, Archaea). Attempts to isolate the epibionts failed and, therefore, growth experiments incorporating the antibiotic anisomycin were conducted in order to investigate the potential symbiotic relationship between <i>P. salinarum</i> and the epibionts. The results suggested an obligate symbiosis between the two organisms and revealed the first symbiotic representative of the <i>Halobacteria</i>. Based on the phylogenetic analyses and growth experiments we propose the classification of this novel organism in a new genus, with the taxon name “<i>Candidatus</i> Haloectosymbiotes riaformosensis”.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"\"<i>Candidatus</i> Haloectosymbiotes riaformosensis\" (Halobacteriaceae), an archaeal ectosymbiont of the hypersaline ciliate <i>Platynematum salinarum</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:10.414113","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Adaptation; Ciliate; Epibiont; Halobacteriaceae; Halophile; Solarsaltern; Symbioses","OtherDescriptors":null,"Notes":null,"AnaPub":2014,"MonPub":null,"DateUpdate":"2017-09-21","DateCreate":"2017-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000337071700002","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.syapm.2014.01.001"},"refs":null,"anarec":{"AnaID":289296,"PubliDate":2014,"Pagination":"244-251","XtraPublOfAnaID":null,"ISBN":null,"Volume":"37","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43944,"SerRR":"Systematic and Applied Microbiology. Elsevier: Jena.  ISSN 0723-2020; e-ISSN 1618-0984","StandardTitleSer":"Systematic and Applied Microbiology","ISSN":"0723-2020","AbbrevSer":"Syst. Appl. Microbiol.","StandardTitleMon":null,"StartPage":244,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Filker","Firstname":"Sabine","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281333,"AutID":271611,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kaiser","Firstname":"Marina","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281333,"AutID":270830,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Rosselló-Mora","Firstname":"Ramón","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281333,"AutID":57136,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dunthorn","Firstname":"Micah","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281333,"AutID":166125,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lax","Firstname":"Gordon","Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281333,"AutID":270832,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Stoeck","Firstname":"Thorsten","Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281333,"AutID":39988,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":307115,"LibID":36,"BRefID":289296,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"307115","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Jena"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTermPath":"Marine Sciences"},{"ThesaurusTerm":"Marine Genomics","ThestID":169586,"Acronym":"KTPS","ThesTermPath":"Marine Sciences > Marine Genomics"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTermPath":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTermPath":"Scientific Publication"}],"thestermsASFA":[{"ThesaurusTerm":"Marine Genomics"},{"ThesaurusTerm":"Marine Sciences"},{"ThesaurusTerm":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":[{"ProID":4198,"Acronym":"ASSEMBLE","ProStandardTitle":"Association of European marine biological laboratories","BYear":2009,"BMonth":3,"EYear":2013,"EMonth":2,"FP7Code":"A-2008","RelID":null,"Relation":null,"DutchTerm":null}],"Physdatasets":null,"spcols":{"990":{"SpName":"Assemble","SpColID":990,"ParSpColID":null,"TopParID":null,"ShortName":"Assemble","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Assemble"},"951":{"SpName":"AssembleMarine","SpColID":951,"ParSpColID":990,"TopParID":990,"ShortName":"AssembleMarine","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":990,"SpColPath":"Assemble/AssembleMarine"},"950":{"SpName":"EMBRC","SpColID":950,"ParSpColID":null,"TopParID":null,"ShortName":"EMBRC","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"EMBRC"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":281333,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":281333,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.syapm.2014.01.001","externalID":"10.1016/j.syapm.2014.01.001","URLTypeCode":"DOI","URLID":58171,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Marine Genomics","ThestID":169586,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Marine Genomics","DutchTerm":null,"ThestID":169586,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Sciences","DutchTerm":null,"ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","DutchTerm":null,"ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","DutchTerm":null,"ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2017-09-18 22:10:53.193000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2017-09-21 08:23:26.587000","timezone_type":3,"timezone":"Europe/Brussels"}}}