{"refrec":{"BRefID":289310,"RR":"<b>Tiago, D.M.; Marques, C.L.; Roberto, V.P.; Cancela, M.L.; Laizé, V.</b> (2014). Mir-20a regulates <i>in vitro</i> mineralization and BMP signaling pathway by targeting BMP-2 transcript in fish. <i>Arch. Biochem. Biophys. 543</i>: 23-30. <a href=\"https://dx.doi.org/10.1016/j.abb.2013.12.009\" target=\"_blank\">https://dx.doi.org/10.1016/j.abb.2013.12.009</a>","BEntID":281347,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Arch. Biochem. Biophys. 543</i>: 23-30. <a href=\"https://dx.doi.org/10.1016/j.abb.2013.12.009\" target=\"_blank\">https://dx.doi.org/10.1016/j.abb.2013.12.009</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tiago, D.M.; Marques, C.L.; Roberto, V.P.; Cancela, M.L.; Laizé, V.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tiago, D.M. <i>et al.</i>","Englishabstract":"MicroRNAs (miRNAs) are important regulators of vertebrate development but their role during skeletogenesis remains unknown. In this regard, we investigated the mineralogenic activity of miR-20a, a miRNA associated with osteogenesis, in fish bone-derived cells. Expression of miR-20a was up-regulated during differentiation and its overexpression inhibited mineralization, suggesting a role in fish tissue calcification. In this regard, a conserved miR-20a binding site was identified in bone morphogenetic protein 2 (BMP-2) 3'UTR and its functionality was evidenced through luciferase assays, and further confirmed by western-blot and qPCR. Type II BMP receptor (BMPR2) is also targeted by miR-20a in mammalian systems and evidence was collected for the presence of a binding site in fish sequences. We propose that miR-20a is a regulator of BMP pathway through specific action on BMP-2 and possibly BMPR2. Overexpression of miR-20a was also shown to up-regulate matrix Gla protein (MGP) transcript, a physiological inhibitor of calcification previously found to form a complex with BMP-2. We propose that MGP may play a role in the anti-mineralogenic effect promoted by miR-20a by decreasing availability of BMP-2. 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