{"refrec":{"BRefID":289341,"RR":"<b>Tait, K.; Havenhand, J.</b> (2013). Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in <i>Balanus improvisus</i> cyprid settlement. <i>Mol. Ecol. 22(9)</i>: 2588-2602. <a href=\"https://dx.doi.org/10.1111/mec.12273\" target=\"_blank\">https://dx.doi.org/10.1111/mec.12273</a>","BEntID":281378,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mol. Ecol. 22(9)</i>: 2588-2602. <a href=\"https://dx.doi.org/10.1111/mec.12273\" target=\"_blank\">https://dx.doi.org/10.1111/mec.12273</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tait, K.; Havenhand, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tait, K.; Havenhand, J.","Englishabstract":"Increased settlement on bacterial biofilms has been demonstrated for a number of marine invertebrate larvae, but the nature of the cue(s) responsible is not well understood. We tested the hypothesis that the bay barnacle <i>Balanus improvisus</i> utilizes the bacterial signal molecules N-acylhomoserine lactones (AHLs) as a cue for the selection of sites for permanent attachment. Single species biofilms of the AHL-producing bacteria <i>Vibrio anguillarum</i>,<i> Aeromonas hydrophila</i> and <i>Sulfitobacter</i> sp. BR1 were attractive to settling cypris larvae of <i>B. improvisus</i>. However, when AHL production was inactivated, either by mutation of the AHL synthetic genes or by expression of an AHL-degrading gene (aiiA), the ability of the bacteria to attract cyprids was abolished. In addition, cyprids actively explored biofilms of <i>E. coli</i> expressing the recombinant AHL synthase genes <i>luxI</i> from <i>Vibrio fischeri</i> (3-oxo-C6-HSL), <i>rhlI</i> from <i>Pseudomonas aeruginosa</i> (C4-HSL/C6-HSL), <i>vanI</i> from <i>V. anguillarum</i> (3-oxo-C10-HSL) and <i>sulI</i> from <i>Sulfitobacter</i> sp. BR1 (C4-HSL, 3-hydroxy-C6-HSL, C8-HSL and 3-hydroxy-C10-HSL), but not <i>E. coli</i> that did not produce AHLs. Finally, synthetic AHLs (C8-HSL, 3-oxo-C10-HSL and C12-HSL) at concentrations similar to those found within natural biofilms (5 μm) resulted in increased cyprid settlement. Thus, <i>B. improvisus</i> cypris exploration of and settlement on biofilms appears to be mediated by AHL-signalling bacteria in the laboratory. This adds to our understanding of how quorum sensing inhibition may be used as for biofouling control. Nonetheless, the significance of our results for larvae settling naturally in the field, and the mechanisms that underlay the observed responses to AHLs, is as yet unknown.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in <i>Balanus improvisus</i> cyprid settlement","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:31:58.963824","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Balanus improvisus; biofilm; N-acylhomoserine lactone; quorum sensing;settlement response","OtherDescriptors":null,"Notes":null,"AnaPub":2013,"MonPub":null,"DateUpdate":"2017-09-20","DateCreate":"2017-09-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000318111100021","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/mec.12273"},"refs":null,"anarec":{"AnaID":289341,"PubliDate":2013,"Pagination":"2588-2602","XtraPublOfAnaID":null,"ISBN":null,"Volume":"22","Issue":"9","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45425,"SerRR":"Molecular Ecology. Blackwell: Oxford.  ISSN 0962-1083; e-ISSN 1365-294X","StandardTitleSer":"Molecular Ecology","ISSN":"0962-1083","AbbrevSer":"Mol. Ecol.","StandardTitleMon":null,"StartPage":2588,"Pages":15,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Tait","Firstname":"Karen","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281378,"AutID":271002,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Havenhand","Firstname":"Jon","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":281378,"AutID":193187,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":307019,"LibID":36,"BRefID":289341,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"307019","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell","City":"Oxford"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Biofouling","ThestID":169568,"Acronym":"KTPS","ThesTermPath":"Aquaculture > Biofouling"},{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTermPath":"Marine Sciences"},{"ThesaurusTerm":"Marine Genomics","ThestID":169586,"Acronym":"KTPS","ThesTermPath":"Marine Sciences > Marine Genomics"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTermPath":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTermPath":"Scientific Publication"}],"thestermsASFA":[{"ThesaurusTerm":"Biofouling"},{"ThesaurusTerm":"Marine Genomics"},{"ThesaurusTerm":"Marine Sciences"},{"ThesaurusTerm":"Scientific Community"},{"ThesaurusTerm":"Scientific Publication"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":[{"ProID":4198,"Acronym":"ASSEMBLE","ProStandardTitle":"Association of European marine biological laboratories","BYear":2009,"BMonth":3,"EYear":2013,"EMonth":2,"FP7Code":"A-2008","RelID":null,"Relation":null,"DutchTerm":null}],"Physdatasets":null,"spcols":{"990":{"SpName":"Assemble","SpColID":990,"ParSpColID":null,"TopParID":null,"ShortName":"Assemble","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"Assemble"},"951":{"SpName":"AssembleMarine","SpColID":951,"ParSpColID":990,"TopParID":990,"ShortName":"AssembleMarine","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":990,"SpColPath":"Assemble/AssembleMarine"},"950":{"SpName":"EMBRC","SpColID":950,"ParSpColID":null,"TopParID":null,"ShortName":"EMBRC","URLLocation":null,"LibID":null,"OpenRepoFlag":0,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"EMBRC"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":281378,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":281378,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1111/mec.12273","externalID":"10.1111/mec.12273","URLTypeCode":"DOI","URLID":58212,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Biofouling","ThestID":169568,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Genomics","ThestID":169586,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Sciences","ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Biofouling","DutchTerm":null,"ThestID":169568,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Genomics","DutchTerm":null,"ThestID":169586,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Marine Sciences","DutchTerm":null,"ThestID":169563,"Acronym":"KTPS","ThesTypID":10,"ThesType":"Assemble Sectors"},{"ThesaurusTerm":"Scientific Community","DutchTerm":null,"ThestID":169557,"Acronym":"KTPUT","ThesTypID":9,"ThesType":"Assemble User Types"},{"ThesaurusTerm":"Scientific Publication","DutchTerm":null,"ThestID":169548,"Acronym":"KTPKO","ThesTypID":8,"ThesType":"Assemble KO Types"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2017-09-18 22:10:53.193000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2017-09-20 08:31:24.910000","timezone_type":3,"timezone":"Europe/Brussels"}}}