{"refrec":{"BRefID":2894,"RR":"<b>Verpraet, R.; Chair, M.; Léger, P.; Nelis, H.; Sorgeloos, P.; De Leenheer, A.P.</b> (1992). Live-food mediated drug delivery as a tool for disease treatment in larviculture: the enrichment of therapeutics in rotifers and <i>Artemia</i> nauplii. <i>Aquacult. Eng. 11</i>: 133-139. <a href=\"https://dx.doi.org/10.1016/0144-8609(92)90015-P\" target=\"_blank\">https://dx.doi.org/10.1016/0144-8609(92)90015-P</a>","BEntID":2894,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquacult. Eng. 11</i>: 133-139. <a href=\"https://dx.doi.org/10.1016/0144-8609(92)90015-P\" target=\"_blank\">https://dx.doi.org/10.1016/0144-8609(92)90015-P</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Verpraet, R.; Chair, M.; Léger, P.; Nelis, H.; Sorgeloos, P.; De Leenheer, A.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Verpraet, R. <i>et al.</i>","Englishabstract":"The live food enrichment technique using emulsions for nutritional deficiencies in rotifers and <i>Artemia</i> was further investigated as a tool for transferring therapeutics through the food chain. <i>Artemia</i> nauplii and the rotifer <i>Brachionus plicatilis</i> were enriched with a self-emulsifying concentrate in which the therapeutic mixture trimetoprim: sulfamethoxazole was incorporated. An analytical method and protocol for the quantification of trimethoprim and sulfamethoxazole in a heterogeneous biological matrix, such as <i>Artemia</i> , was developed using High Performance Liquid Chromatography. When applying a 24h enrichment period, the accumulation of both drugs in <i>Artemia</i> nauplii appeared to be linearly related to the concentration of the drugs in the enrichment product. Values of approximately 790 mg/kg of total drugs (protein basis; or 290 mg/kg on dry weight basis) could be obtained. In rotifers, the level of total therapeutics reached a value of about 116,mg/kg (protein basis) after 6h of enrichment. The ratio of the two drugs detected in rotifers was different from the one found in <i>Artemia</i> , suggesting different accumulation kinetics.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Live-food mediated drug delivery as a tool for disease treatment in larviculture: the enrichment of therapeutics in rotifers and <i>Artemia</i> nauplii","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-29 01:30:46.480966","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1992,"MonPub":null,"DateUpdate":"2001-11-28","DateCreate":"2000-08-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1992JX06400005","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0144-8609(92)90015-P"},"refs":null,"anarec":{"AnaID":2894,"PubliDate":1992,"Pagination":"133-139","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42374,"SerRR":"Aquacultural engineering. Elsevier: London.  ISSN 0144-8609; e-ISSN 1873-5614","StandardTitleSer":"Aquacultural engineering","ISSN":"0144-8609","AbbrevSer":"Aquacult. Eng.","StandardTitleMon":null,"StartPage":133,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"Univ. Ghent","Email":null,"Line2":null,"EnvName":"Belgium"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Verpraet","Firstname":null,"Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":2894,"AutID":4894,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chair","Firstname":null,"Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":2894,"AutID":4333,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Léger","Firstname":"Philippe","Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":2894,"AutID":4609,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Nelis","Firstname":null,"Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":2894,"AutID":4357,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":592,"InsID":null},{"AutName":"Sorgeloos","Firstname":"Patrick","Initials":"P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":2894,"AutID":89,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":35,"InsID":null},{"AutName":"De Leenheer","Firstname":"Andreas","Initials":"A.P.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":2894,"AutID":639,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":4667,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":135408,"LibID":36,"BRefID":2894,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"135408","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Aquaculture systems","ThestID":488,"Acronym":"ASFA","ThesTermPath":"Aquaculture systems"},{"ThesaurusTerm":"Disease control","ThestID":2349,"Acronym":"ASFA","ThesTermPath":"Control > Disease control"},{"ThesaurusTerm":"Drugs","ThestID":2522,"Acronym":"ASFA","ThesTermPath":"Drugs"},{"ThesaurusTerm":"Food chains","ThestID":3391,"Acronym":"ASFA","ThesTermPath":"Food webs > Food chains"},{"ThesaurusTerm":"Therapy","ThestID":8482,"Acronym":"ASFA","ThesTermPath":"Therapy"}],"thestermsASFA":[{"ThesaurusTerm":"Aquaculture systems"},{"ThesaurusTerm":"Disease control"},{"ThesaurusTerm":"Drugs"},{"ThesaurusTerm":"Food chains"},{"ThesaurusTerm":"Therapy"}],"taxtermsASFA":[{"TaxTerm":"Artemia"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":2894,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":2894,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/0144-8609(92)90015-P","externalID":"10.1016/0144-8609(92)90015-P","URLTypeCode":"DOI","URLID":110245,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Aquaculture systems","ThestID":488,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Disease control","ThestID":2349,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Drugs","ThestID":2522,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Food chains","ThestID":3391,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Therapy","ThestID":8482,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Artemia","AphiaID":480245,"TaxtID":23717}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Aquaculture systems","DutchTerm":null,"ThestID":488,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Disease control","DutchTerm":null,"ThestID":2349,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Drugs","DutchTerm":"Drugs","ThestID":2522,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Food chains","DutchTerm":"Voedselketens","ThestID":3391,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Therapy","DutchTerm":null,"ThestID":8482,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Artemia","DutchTerm":"Pekelkreeftjes","AphiaID":480245,"TaxtID":23717}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"VLIZ2000\\stefaan","newSesDate":{"date":"2000-08-24 19:15:10.000000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"VLIZ2000\\Myriam","updSesDate":{"date":"2001-11-28 13:42:21.523000","timezone_type":3,"timezone":"Europe/Brussels"}}}