{"refrec":{"BRefID":291062,"RR":"<b>Hagedorn, M.; Carter, V.L.; Henley, E.M.; van Oppen, M.J.H.; Hobbs, R.; Spindler, R.E.</b> (2017). Producing coral offspring with cryopreserved sperm: A tool for coral reef restoration. <i>NPG Scientific Reports 7(1)</i>: 9 pp. <a href=\"https://dx.doi.org/10.1038/s41598-017-14644-x\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-017-14644-x</a>","BEntID":283105,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 7(1)</i>: 9 pp. <a href=\"https://dx.doi.org/10.1038/s41598-017-14644-x\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-017-14644-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hagedorn, M.; Carter, V.L.; Henley, E.M.; van Oppen, M.J.H.; Hobbs, R.; Spindler, R.E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hagedorn, M. <i>et al.</i>","Englishabstract":"Cryopreservation is an important conservation tool, which may help reef-building coral survive. However, scaling-up from small, laboratory-sized experiments to higher-throughput restoration is a major challenge. To be an effective restoration tool, the cryopreservation methods and husbandry to produce new offspring must be defined. This study examined small and larger-scale in vitro reproduction and settlement for <i>Acropora tenuis</i> and <i>Acropora millepora</i> and found that: 1) cryopreservation of coral sperm reduced sperm motility and fertilization success in half, thus fresh sperm, capable of becoming highly motile, is key; 2) the sperm-to-egg ratio and the concentration of the cryoprotectant treatments affected fertilization success in small- and larger-scale reproduction trials using cryopreserved sperm (p < 0.05); 3) cryopreservation did not affect settlement success, as larvae produced with fresh or cryopreserved sperm had the same settlement success (p > 0.05); and 4) the residence time of the sperm within the bank was not important as the fertilization success of sperm frozen for less than 1 month was similar to that frozen up to 2 years (p > 0.05). These results described the first settlement for coral larvae produced from cryopreserved sperm and established important ground-work principles for the use of cryopreserved coral sperm for future reef restoration efforts.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Producing coral offspring with cryopreserved sperm: A tool for coral reef restoration","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-24 01:31:47.449163","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2017,"MonPub":null,"DateUpdate":"2018-02-13","DateCreate":"2017-11-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000414231000033","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-017-14644-x"},"refs":null,"anarec":{"AnaID":291062,"PubliDate":2017,"Pagination":"9 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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