{"refrec":{"BRefID":293270,"RR":"<b>Vandegehuchte, M.B.; Lemière, F.; Vanhaecke, L.; Vanden Berghe, W.; Janssen, C.R.</b> (2010). Direct and transgenerational impact on <i>Daphnia magna</i> of chemicals with a known effect on DNA methylation. <i>Comp. Biochem. Physiol. C-Toxicol. Pharmacol. 151(3)</i>: 278-285. <a href=\"https://dx.doi.org/10.1016/j.cbpc.2009.11.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.cbpc.2009.11.007</a>","BEntID":285324,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Comp. Biochem. Physiol. C-Toxicol. Pharmacol. 151(3)</i>: 278-285. <a href=\"https://dx.doi.org/10.1016/j.cbpc.2009.11.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.cbpc.2009.11.007</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vandegehuchte, M.B.; Lemière, F.; Vanhaecke, L.; Vanden Berghe, W.; Janssen, C.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vandegehuchte, M.B. <i>et al.</i>","Englishabstract":"The purpose of this study is to investigate (1) the induction of epigenetic effects in the crustacean <i>Daphnia magna</i> using DNA methylation as an epigenetic mark and (2) the potential stable transfer of such an epigenetic effect to non-exposed subsequent generations. Daphnids were exposed to chemical substances known to affect DNA methylation in mammals: vinclozolin, 5-azacytidine, 2′-deoxy-5-azacytidine, genistein and biochanin A. Effects on overall DNA cytosine methylation, body length and reproduction were evaluated in 21 day experiments. Using a multi-generational experimental design these endpoints were also evaluated in the F<sub>1</sub> and F<sub>2</sub> generation of both exposed and non-exposed offspring from F<sub>0</sub> daphnids exposed to 5-azacytidine, genistein or vinclozolin. A reduction in DNA methylation was consistently observed in daphnids exposed to vinclozolin and 5-azacytidine. Only in organisms exposed to 5-azacytidine was this effect transferred to the two subsequent non-exposed generations. A concurrent reduction in body length at day 7 was observed in these treatments. For the first time, exposure to environmental chemicals was shown to affect DNA methylation in the parental generation of <i>D. magna</i>. We also demonstrated a transgenerational alteration in an epigenetic system in <i>D. magna</i>, which indicates the possibility of transgenerational inheritance of environment-induced epigenetic changes in non-exposed subsequent generations.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Direct and transgenerational impact on <i>Daphnia magna</i> of chemicals with a known effect on DNA methylation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:13.101726","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"2'-deoxy-5-azacytidine, 5-azacytidine, Biochanin A, Ecotoxicology, Epigenetics, Genistein, Inheritance, Vinclozolin","OtherDescriptors":null,"Notes":null,"AnaPub":2010,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2018-03-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000275627400002","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.cbpc.2009.11.007"},"refs":null,"anarec":{"AnaID":293270,"PubliDate":2010,"Pagination":"278-285","XtraPublOfAnaID":null,"ISBN":null,"Volume":"151","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":193390,"SerRR":"Comparative Biochemistry and Physiology. 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