{"refrec":{"BRefID":293633,"RR":"<b>Bogaert, K.A.; Beeckman, T.; De Clerck, O.</b> (2017). Egg activation-triggered shape change in the <i>Dictyota dichotoma</i> (Phaeophyceae) zygote is actin-myosin and secretion dependent. <i>Ann. Bot. 120(4)</i>: 529-538. <a href=\"https://dx.doi.org/10.1093/aob/mcx085\" target=\"_blank\">https://dx.doi.org/10.1093/aob/mcx085</a>","BEntID":285697,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Ann. Bot. 120(4)</i>: 529-538. <a href=\"https://dx.doi.org/10.1093/aob/mcx085\" target=\"_blank\">https://dx.doi.org/10.1093/aob/mcx085</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bogaert, K.A.; Beeckman, T.; De Clerck, O.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bogaert, K.A. <i>et al.</i>","Englishabstract":"<strong>Background and Aims</strong> Cellular morphogenesis in land plants and brown algae is typically a slow process involving growth established by an interplay of turgor pressure and cell wall rigidity. However, a recent study showed that zygotes of the brown alga <i>Dictyota dichotoma</i> undergo a rapid shape change from a sphere to an elongated spheroid in about 90 s, establishing the first body axis.</p><p><strong>Methods</strong> Using a combination of pharmacology, staining techniques, membrane depolarization and microscopy techniques (brightfield, transmission electron microscopy and confocal laser scanning microscopy), egg activation and the shape change of the egg cell of <i>D. dichotoma</i> was studied.</p><p><strong>Key Results</strong> It was established that elongation of the zygote does not involve growth, i.e. a positive change in size. The elongation is dependent on F-actin and myosin but independent of microtubules. Secretion was also found to be necessary for elongation after addition of brefeldin A. Moreover, a temporal correlation between extracellular matrix secretion and elongation was observed. Ionomycin and high potassium seawater are capable of triggering the onset of elongation, suggesting a role for membrane depolarization and calcium influx in the signalling mechanism. The elongated cells are shorter in the presence of ionomycin, suggesting a role for calcium in elongation.</p><p><strong>Conclusions</strong> A model is proposed in which the fast elongation of the fertilized egg in <i>Dictyota</i> is accomplished by a force generated by F-actin and myosin, regulated by cytoplasmic calcium concentrations and by secretion during elongation lowering the antagonistic force. The finding of early extracellular matrix secretion, membrane depolarization and ionophore-triggered egg activation suggest significant differences in the mechanism of egg activation signalling between <i>D. dichotoma</i> and the oogamous brown algal model system <i>Fucus</i>.</p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Egg activation-triggered shape change in the <i>Dictyota dichotoma</i> (Phaeophyceae) zygote is actin-myosin and secretion dependent","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-04 01:32:11.752044","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Actin; brown algae; cell polarization; cell wall; Dictyotales; Dictyotadichotoma; Dictyotales; egg activation; morphogenesis; secretion; spermblock; zygote","OtherDescriptors":null,"Notes":null,"AnaPub":2017,"MonPub":null,"DateUpdate":"2019-07-08","DateCreate":"2018-03-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000413400400009","VABBcode":null,"OpenAcc":0,"DOI":"10.1093/aob/mcx085"},"refs":null,"anarec":{"AnaID":293633,"PubliDate":2017,"Pagination":"529-538","XtraPublOfAnaID":null,"ISBN":null,"Volume":"120","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42136,"SerRR":"Annals of Botany. Academic Press: London.  ISSN 0305-7364; e-ISSN 1095-8290","StandardTitleSer":"Annals of Botany","ISSN":"0305-7364","AbbrevSer":"Ann. Bot.","StandardTitleMon":null,"StartPage":529,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Bogaert","Firstname":"Kenny","Initials":"K.A.","Affiliation":"Department of Biology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285697,"AutID":320394,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie","ORCID":"0000-0003-1482-7618","PersID":26850,"InsID":91},{"AutName":"Beeckman","Firstname":"Tom","Initials":"T.","Affiliation":"VIB-UGent Center for Plant Systems Biology","Discriminator":null,"CorporateFlag":0,"BEntID":285697,"AutID":318268,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"PSB","InsFSN":"Flanders Institute for Biotechnology; VIB-Ugent Center for Plant Systems Biology","ORCID":"0000-0001-8656-2060","PersID":295,"InsID":7968},{"AutName":"De Clerck","Firstname":"Olivier","Initials":"O.","Affiliation":"Department of Biology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285697,"AutID":258600,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie","ORCID":"0000-0002-3699-8402","PersID":2518,"InsID":91}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":332357,"LibID":36,"BRefID":293633,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"332357","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open Marien Archief","URL":null,"ClassifID":235,"Classification":"Open Marine Archive","ReqLink":null,"ClassifTypID":5,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":0,"InternalReq":0,"LoggedInReq":0,"Disclaimer":"Disclaimer_OMA","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Academic Press","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Dictyota dichotoma"},{"TaxTerm":"Dictyotales"},{"TaxTerm":"Phaeophyceae"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":285697,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":285697,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1093/aob/mcx085","externalID":"10.1093/aob/mcx085","URLTypeCode":"DOI","URLID":62531,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Dictyota dichotoma","AphiaID":145367,"TaxtID":39282},{"TaxTerm":"Dictyotales","AphiaID":839,"TaxtID":15088},{"TaxTerm":"Phaeophyceae [brown algae]","AphiaID":830,"TaxtID":14883}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Dictyota dichotoma","DutchTerm":null,"AphiaID":145367,"TaxtID":39282},{"TaxTerm":"Dictyotales","DutchTerm":null,"AphiaID":839,"TaxtID":15088},{"TaxTerm":"Phaeophyceae","DutchTerm":"Bruinwieren","AphiaID":830,"TaxtID":14883}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2018-03-12 21:15:00.680000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2019-07-08 10:38:16.760000","timezone_type":3,"timezone":"Europe/Brussels"}}}