{"refrec":{"BRefID":293649,"RR":"<b>Laranja, J.L.Q.; Amar, E.C.; Ludevese-Pascual, G.L.; Niu, Y.; Geaga, M.J.; De Schryver, P.; Bossier, P.</b> (2017). A probiotic <i>Bacillus</i> strain containing amorphous poly-beta-hydroxybutyrate (PHB) stimulates the innate immune response of <i>Penaeus monodon</i> postlarvae. <i>Fish Shellfish Immunol. 68</i>: 202-210. <a href=\"https://dx.doi.org/10.1016/j.fsi.2017.07.023\" target=\"_blank\">https://dx.doi.org/10.1016/j.fsi.2017.07.023</a>","BEntID":285713,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Fish Shellfish Immunol. 68</i>: 202-210. <a href=\"https://dx.doi.org/10.1016/j.fsi.2017.07.023\" target=\"_blank\">https://dx.doi.org/10.1016/j.fsi.2017.07.023</a>","DocTypID":9,"DocType":"Map","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Laranja, J.L.Q.; Amar, E.C.; Ludevese-Pascual, G.L.; Niu, Y.; Geaga, M.J.; De Schryver, P.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Laranja, J.L.Q. <i>et al.</i>","Englishabstract":"In this study, the PHB-accumulating <i>Bacillus</i> sp. JL47 strain (capable of accumulating 55% PHB on cell dry weight) was investigated for its effects on the immune response of giant tiger shrimp (<i>Penaeus monodon</i>) postlarvae (PL) before and after the <i>Vibrio campbellii</i> challenge. Briefly, shrimp PL were cultured and fed with <i>Artemia</i> nauplii enriched with <i>Bacillus</i> sp. JL47. Shrimp receiving the <i>Artemia</i> nauplii without JL47 enrichment were used as control. After 15 days of feeding, the shrimp were challenged with pathogenic <i>V. campbellii</i> LMG 21363 at 10<sup>6</sup> cells mL<sup>−1</sup> by immersion. Relative expression of the immune related genes encoding for prophenoloxidase (proPO), transglutaminase(TGase) and heat shock protein 70 (Hsp70) in the shrimp were measured before (0 h) and after (3, 6, 9, 12, 24 h) the <i>Vibrio</i> challenge by quantitative real-time PCR using β-actin as the reference gene. The expressions of TGase and proPO were significantly up-regulated (p < 0.05) within 9 h and 12 h, respectively after challenge in shrimp receiving the <i>Bacillus</i> sp. JL47 as compared to the challenged and non-challenged controls. Hsp70 expression was significantly increased (p < 0.05) at 3 h post-challenge in all challenged shrimp. Interestingly, proPO and TGase genes were significantly up-regulated (p < 0.05) in <i>Bacillus</i> sp. JL47 treated shrimp even before the <i>Vibrio</i> challenge was applied. No up-regulation in the Hsp70 gene, however, was observed under these conditions. The data suggest that the protective effect of the PHB-accumulating <i>Bacillus</i> sp. JL47 in shrimp was due to its capacity to stimulate the innate immune related genes of the shrimp, specifically the proPO and TGase genes. The application of probiotic <i>Bacillus</i> species, capable of accumulating a significant amount of PHB, is suggested as potential immunostimulatory strategy for aquaculture.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A probiotic <i>Bacillus</i> strain containing amorphous poly-beta-hydroxybutyrate (PHB) stimulates the innate immune response of <i>Penaeus monodon</i> postlarvae","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:32:39.877711","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Amorphous PHB; Probiotic Bacillus; Prophenoloxidase; Transglutaminase;Heat shock protein 70; Penaeus monodon postlarvae","OtherDescriptors":null,"Notes":null,"AnaPub":2017,"MonPub":null,"DateUpdate":"2019-07-09","DateCreate":"2018-03-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000411299500021","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.fsi.2017.07.023"},"refs":null,"anarec":{"AnaID":293649,"PubliDate":2017,"Pagination":"202-210","XtraPublOfAnaID":null,"ISBN":null,"Volume":"68","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45027,"SerRR":"Fish & Shellfish Immunology. 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