{"refrec":{"BRefID":29365,"RR":"<b>Köllner, B.; Kotterba, G.</b> (2002). Temperature dependent activation of leucocyte populations of rainbow trout, <i>Oncorhynchus mykiss</i>, after intraperitoneal immunisation with <i>Aeromonas salmonicida</i>. <i>Fish Shellfish Immunol. 12(1)</i>: 35-48. <a href=\"https://dx.doi.org/10.1006/fsim.2001.0352\" target=\"_blank\">https://dx.doi.org/10.1006/fsim.2001.0352</a>","BEntID":29365,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Fish Shellfish Immunol. 12(1)</i>: 35-48. <a href=\"https://dx.doi.org/10.1006/fsim.2001.0352\" target=\"_blank\">https://dx.doi.org/10.1006/fsim.2001.0352</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Köllner, B.; Kotterba, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Köllner, B.; Kotterba, G.","Englishabstract":"The temperature dependence of in vivo activation of rainbow  trout <i>Oncorhynchus mykiss</i>, leucocyte populations after intraperitoneal injection (i.p.) of fish with a T-cell independent antigen <i>Aeromonas salmonicida</i> (strain MT423) was investigated using a proliferation assay and flow cytometric analysis with mab specific for trout leucocyte surface markers. In trout kept at 15-17° C a prominent activation of blood and spleen leucocytes was found. Also, drastic changes of the percentage of the leucocyte populations in blood and spleen occurred: the amount of monocytes in the blood increased between day 2 and day 7 post injection (p.i.), whereas in spleen the amount of monocytes stayed at a high level (~35%) after a depression between day 4 and day 7 p.i. The percentage of B-lymphocytes was increased first in spleen and then in blood. The percentage of granulocytes in blood was elevated during the whole experiment compared to control fish. In trout kept at 10-12° C only blood leucocytes showed a weak activation after i.p. injection of <i>A. salmonicida</i>, whereas spleen leucocytes showed nearly no reaction. Only the percentage of granulocytes in the blood (day 2-14 p.i.) and of monocytes in the spleen (day 2 and day 8 p.i.) was changed compared to phosphate buffered saline (PBS)-injected fish. However, the development of <i>A. salmonicida</i> specific antibodies was contrary to the cellular reaction. Whereas antibodies could first be detected after 16-18 days p.i. in both groups the amount of antibodies was significantly higher in sera of trout kept at 10-12° C at day 22 and day 28 p.i. than in sera of trout kept at 15-17° C. These results indicate stronger <i>A. salmonicida</i> induced activation of monocytes, granulocytes and B-lymphocytes at higher temperature. However, the development of a specific antibody response against <i>A. salmonicida</i> seemed to be more effective at lower temperatures.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Temperature dependent activation of leucocyte populations of rainbow trout, <i>Oncorhynchus mykiss</i>, after intraperitoneal immunisation with <i>Aeromonas salmonicida</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-05 01:30:43.203386","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"rainbow trout, immune reaction, in vivo activation, leucocyte populations, <i>Aeromonas salmonicida</i>","Notes":null,"AnaPub":2002,"MonPub":null,"DateUpdate":"2002-11-13","DateCreate":"2002-11-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000173477000003","VABBcode":null,"OpenAcc":0,"DOI":"10.1006/fsim.2001.0352"},"refs":null,"anarec":{"AnaID":29365,"PubliDate":2002,"Pagination":"35-48","XtraPublOfAnaID":null,"ISBN":null,"Volume":"12","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45027,"SerRR":"Fish & Shellfish Immunology. Academic Press: London; New York.  ISSN 1050-4648; e-ISSN 1095-9947","StandardTitleSer":"Fish & Shellfish Immunology","ISSN":"1050-4648","AbbrevSer":"Fish Shellfish Immunol.","StandardTitleMon":null,"StartPage":35,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"D-17498, Insel Riems","Email":"bernd.koellner@rie.bfav.de","Line2":null,"EnvName":"Germany"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Köllner","Firstname":null,"Initials":"B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":29365,"AutID":31979,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":1,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kotterba","Firstname":null,"Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":29365,"AutID":31980,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Academic Press","City":"London; New York"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Leukicytes","ThestID":4685,"Acronym":"ASFA","ThesTermPath":"Cells > Blood cells > Leukicytes"},{"ThesaurusTerm":"Rainbow trout","ThestID":139821,"Acronym":"CAB","ThesTermPath":"Fishes > Osteichthyes > Salmoniformes > Salmonidae > Salmo > Freshwater fishes > Rainbow trout"},{"ThesaurusTerm":"Population number","ThestID":6442,"Acronym":"ASFA","ThesTermPath":"Population characteristics > Population number"},{"ThesaurusTerm":"Immunity","ThestID":4247,"Acronym":"ASFA","ThesTermPath":"Properties > Biological properties > Immunity"}],"thestermsASFA":[{"ThesaurusTerm":"Immunity"},{"ThesaurusTerm":"Leukicytes"},{"ThesaurusTerm":"Population number"},{"ThesaurusTerm":"Rainbow trout"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":29365,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1006/fsim.2001.0352","externalID":"10.1006/fsim.2001.0352","URLTypeCode":"DOI","URLID":109825,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Immunity","ThestID":4247,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Leukicytes","ThestID":4685,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Population number","ThestID":6442,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Rainbow trout","ThestID":139821,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxterms":null,"geoterms":null,"othterms":[{"OtherTerm":"<i>aeromonas salmonicida</i>","OthtID":10571},{"OtherTerm":"Immune reaction","OthtID":6117},{"OtherTerm":"In vivo activation","OthtID":8241},{"OtherTerm":"Leucocyte populations","OthtID":9904}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Immunity","DutchTerm":"Immuniteit","ThestID":4247,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Leukicytes","DutchTerm":null,"ThestID":4685,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Population number","DutchTerm":"Populatieaantal","ThestID":6442,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Rainbow trout","DutchTerm":null,"ThestID":139821,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"<i>aeromonas salmonicida</i>","DutchTerm":null,"OthtID":10571},{"OtherTerm":"Immune reaction","DutchTerm":null,"OthtID":6117},{"OtherTerm":"In vivo activation","DutchTerm":null,"OthtID":8241},{"OtherTerm":"Leucocyte populations","DutchTerm":null,"OthtID":9904}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2002-11-13 08:54:50.403000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2002-11-13 08:54:50.403000","timezone_type":3,"timezone":"Europe/Brussels"}}}