{"refrec":{"BRefID":293681,"RR":"<b>Cuypers, B.; Vermeylen, S.; Hammerschmid, D.; Trashin, S.; Rahemi, V.; Konijnenberg, A.; De Schutter, A.; Cheng, C.-H.; Giordano, D.; Verde, C.; De Wael, K.; Sobott, F.; Dewilde, S.; Van Doorslaer, S.</b> (2017). Antarctic fish versus human cytoglobins - The same but yet so different. <i>J. Inorg. Biochem. 173</i>: 66-78. <a href=\"https://dx.doi.org/10.1016/j.jinorgbio.2017.04.025\" target=\"_blank\">https://dx.doi.org/10.1016/j.jinorgbio.2017.04.025</a>","BEntID":285745,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>J. Inorg. Biochem. 173</i>: 66-78. <a href=\"https://dx.doi.org/10.1016/j.jinorgbio.2017.04.025\" target=\"_blank\">https://dx.doi.org/10.1016/j.jinorgbio.2017.04.025</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Cuypers, B.; Vermeylen, S.; Hammerschmid, D.; Trashin, S.; Rahemi, V.; Konijnenberg, A.; De Schutter, A.; Cheng, C.-H.; Giordano, D.; Verde, C.; De Wael, K.; Sobott, F.; Dewilde, S.; Van Doorslaer, S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Cuypers, B. <i>et al.</i>","Englishabstract":"The cytoglobins of the Antarctic fish Chaenocephalus aceratus and Dissostichus mawsoni have many features in common with human cytoglobin. These cytoglobins are heme proteins in which the ferric and ferrous forms have a characteristic hexacoordination of the heme iron, i.e. axial ligation of two endogenous histidine residues, as confirmed by electron paramagnetic resonance, resonance Raman and optical absorption spectroscopy. The combined spectroscopic analysis revealed only small variations in the heme-pocket structure, in line with the small variations observed for the redox potential. Nevertheless, some striking differences were also discovered. Resonance Raman spectroscopy showed that the stabilization of an exogenous heme ligand, such as CO, occurs differently in human cytoglobin in comparison with Antarctic fish cytoglobins. Furthermore, while it has been extensively reported that human cytoglobin is essentially monomeric and can form an intramolecular disulfide bridge that can influence the ligand binding kinetics, 3D modeling of the Antarctic fish cytoglobins indicates that the cysteine residues are too far apart to form such an intramolecular bridge. Moreover, gel filtration and mass spectrometry reveal the occurrence of non-covalent multimers (up to pentamers) in the Antarctic fish cytoglobins that are formed at low concentrations. Stabilization of these oligomers by disulfide-bridge formation is possible, but not essential. If intermolecular disulfide bridges are formed, they influence the heme-pocket structure, as is shown by EPR measurements.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Antarctic fish versus human cytoglobins - The same but yet so different","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:32:39.924585","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Cytoglobin; Protein expression; Electron paramagnetic resonance;Resonance Raman spectroscopy; Mass spectrometry; Redox","OtherDescriptors":null,"Notes":null,"AnaPub":2017,"MonPub":null,"DateUpdate":"2020-06-26","DateCreate":"2018-03-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000405159600007","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.jinorgbio.2017.04.025"},"refs":null,"anarec":{"AnaID":293681,"PubliDate":2017,"Pagination":"66-78","XtraPublOfAnaID":null,"ISBN":null,"Volume":"173","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":223228,"SerRR":"Journal of Inorganic Biochemistry. Elsevier: New York,.  ISSN 0162-0134; e-ISSN 1873-3344","StandardTitleSer":"Journal of Inorganic Biochemistry","ISSN":"0162-0134","AbbrevSer":"J. Inorg. Biochem.","StandardTitleMon":null,"StartPage":66,"Pages":13,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Cuypers","Firstname":"Bert","Initials":"B.","Affiliation":"BIMEF Laboratory, Department of Physics, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318516,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Antwerpen; Departement Natuurkunde; Onderzoeksgroep Biomedische Fysica","ORCID":null,"PersID":39162,"InsID":571},{"AutName":"Vermeylen","Firstname":"Stijn","Initials":"S.","Affiliation":"PPES Laboratory, Department of Biomedical Sciences, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318517,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hammerschmid","Firstname":"Dietmar","Initials":"D.","Affiliation":"PPES Laboratory, Department of Biomedical Sciences, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318518,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Trashin","Firstname":"Stanislav","Initials":"S.","Affiliation":"AXES Laboratory, Department of Chemistry, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318519,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0002-4089-4251","PersID":33375,"InsID":null},{"AutName":"Rahemi","Firstname":"Vanoushe","Initials":"V.","Affiliation":"AXES Laboratory, Department of Chemistry, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318520,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Konijnenberg","Firstname":"Albert","Initials":"A.","Affiliation":"AXES Laboratory, Department of Chemistry, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318521,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"De Schutter","Firstname":"Amy","Initials":"A.","Affiliation":"BIMEF Laboratory, Department of Physics, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318522,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Antwerpen; Departement Natuurkunde; Onderzoeksgroep Biomedische Fysica","ORCID":null,"PersID":null,"InsID":571},{"AutName":"Cheng","Firstname":"Christina","Initials":"C.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318523,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Giordano","Firstname":"Daniela","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":34060,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Verde","Firstname":"Cinzia","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":77848,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"De Wael","Firstname":"Karolien","Initials":"K.","Affiliation":"AXES Laboratory, Department of Chemistry, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318526,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sobott","Firstname":"Frank","Initials":"F.","Affiliation":"BAMS Laboratory, Department of Chemistry, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318527,"OrderNr":12,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0001-9029-1865","PersID":38812,"InsID":null},{"AutName":"Dewilde","Firstname":"Sylvia","Initials":"S.","Affiliation":"PPES Laboratory, Department of Biomedical Sciences, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318528,"OrderNr":13,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":2626,"InsID":null},{"AutName":"Van Doorslaer","Firstname":"Sabine","Initials":"S.","Affiliation":"BIMEF Laboratory, Department of Physics, University of Antwerp","Discriminator":null,"CorporateFlag":0,"BEntID":285745,"AutID":318529,"OrderNr":14,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Antwerpen; Departement Natuurkunde; Onderzoeksgroep Biomedische Fysica","ORCID":"0000-0002-1685-9202","PersID":34548,"InsID":571}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":332398,"LibID":36,"BRefID":293681,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"332398","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"New York,"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":285745,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":285745,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.jinorgbio.2017.04.025","externalID":"10.1016/j.jinorgbio.2017.04.025","URLTypeCode":"DOI","URLID":62576,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2018-03-12 21:15:00.680000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2020-06-26 07:42:22.443000","timezone_type":3,"timezone":"Europe/Brussels"}}}