{"refrec":{"BRefID":293783,"RR":"<b>Grudniewska, M.; Mouton, S.; Simanov, D.; Beltman, F.; Grelling, M.; De Mulder, K.; Arindrarto, W.; Weissert, P.M.; van der Elst, S.; Berezikov, E.</b> (2016). Transcriptional signatures of somatic neoblasts and germline cells in <i>Macrostomum lignano</i>. <i>eLIFE 5</i>: 23. <a href=\"https://dx.doi.org/10.7554/eLife.20607\" target=\"_blank\">https://dx.doi.org/10.7554/eLife.20607</a>","BEntID":285847,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>eLIFE 5</i>: 23. <a href=\"https://dx.doi.org/10.7554/eLife.20607\" target=\"_blank\">https://dx.doi.org/10.7554/eLife.20607</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Grudniewska, M.; Mouton, S.; Simanov, D.; Beltman, F.; Grelling, M.; De Mulder, K.; Arindrarto, W.; Weissert, P.M.; van der Elst, S.; Berezikov, E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Grudniewska, M. <i>et al.</i>","Englishabstract":"The regeneration-capable flatworm <i>Macrostomum lignano</i> is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as <i>Schmidtea mediterranea</i>. However, information on the transcriptome and markers of stem cells in <i>M. lignano</i> is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of <i>M. lignano</i>, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including <i>Mlig-ddx39</i>, <i>Mlig-rrm1</i>, <i>Mlig-rpa3</i>, <i>Mlig-cdk1</i>, and <i>Mlig-h2a</i>, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated <i>M. lignano</i> transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in <i>M. lignano</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Transcriptional signatures of somatic neoblasts and germline cells in <i>Macrostomum lignano</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-03-29","DateCreate":"2018-03-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000391543200001","VABBcode":null,"OpenAcc":1,"DOI":"10.7554/eLife.20607"},"refs":null,"anarec":{"AnaID":293783,"PubliDate":2016,"Pagination":"23","XtraPublOfAnaID":null,"ISBN":null,"Volume":"5","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":240535,"SerRR":"eLIFE. eLife Sciences Publications: Cambridge.  e-ISSN 2050-084X","StandardTitleSer":"eLIFE","ISSN":null,"AbbrevSer":null,"StandardTitleMon":null,"StartPage":23,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Grudniewska","Firstname":"Magda","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":318029,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Mouton","Firstname":"Stijn","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":318038,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":30144,"InsID":null},{"AutName":"Simanov","Firstname":"Daniil","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":318025,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Beltman","Firstname":"Frank","Initials":"F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":318030,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Grelling","Firstname":"Margriet","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":318028,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"De Mulder","Firstname":"Katrien","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":243045,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":30204,"InsID":null},{"AutName":"Arindrarto","Firstname":"Wibowo","Initials":"W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":319142,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Weissert","Firstname":"Philipp","Initials":"P.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":318036,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"van der Elst","Firstname":"Stefan","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":319143,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Berezikov","Firstname":"Eugene","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285847,"AutID":182473,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":312226,"LibID":36,"BRefID":293783,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"312226","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"eLife Sciences Publications","City":"Cambridge"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":285847,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":285847,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.7554/eLife.20607","externalID":"10.7554/eLife.20607","URLTypeCode":"DOI","URLID":62676,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2018-03-12 21:25:44.307000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2018-03-29 10:39:02.970000","timezone_type":3,"timezone":"Europe/Brussels"}}}