{"refrec":{"BRefID":293794,"RR":"<b>Douglas, T.E.L.; Dokupil, A.; Reczynska, K.; Brackman, G.; Krok-Borkowicz, M.; Keppler, J.K.; Bozic, M.; Van der Voort, P.; Pietryga, K.; Samal, S.K.; Balcaen, L.; van den Bulcke, J.; Van Acker, J.; Vanhaecke, F.; Schwarz, K.; Coenye, T.; Pamula, E.</b> (2016). Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich <i>Ecklonia cava</i> extract Seanol® to endow antibacterial properties and promote mineralization. <i>Biomedical Materials 11(4)</i>: 045015. <a href=\"https://dx.doi.org/10.1088/1748-6041/11/4/045015\" target=\"_blank\">https://dx.doi.org/10.1088/1748-6041/11/4/045015</a>","BEntID":285858,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Biomedical Materials 11(4)</i>: 045015. <a href=\"https://dx.doi.org/10.1088/1748-6041/11/4/045015\" target=\"_blank\">https://dx.doi.org/10.1088/1748-6041/11/4/045015</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Douglas, T.E.L.; Dokupil, A.; Reczynska, K.; Brackman, G.; Krok-Borkowicz, M.; Keppler, J.K.; Bozic, M.; Van der Voort, P.; Pietryga, K.; Samal, S.K.; Balcaen, L.; van den Bulcke, J.; Van Acker, J.; Vanhaecke, F.; Schwarz, K.; Coenye, T.; Pamula, E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Douglas, T.E.L. <i>et al.</i>","Englishabstract":"Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol<sup>®</sup>, a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol<sup>®</sup> enhanced mineralization with CaP and also increased compressive modulus. Seanol<sup>®</sup> and ALP interacted in a non-covalent manner. Release of Seanol<sup>®</sup> occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i>. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich <i>Ecklonia cava</i> extract Seanol® to endow antibacterial properties and promote mineralization","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"polyphenol; hydrogel; mineralization; antibacterial; composite","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2021-09-08","DateCreate":"2018-03-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000383288600016","VABBcode":null,"OpenAcc":0,"DOI":"10.1088/1748-6041/11/4/045015"},"refs":null,"anarec":{"AnaID":293794,"PubliDate":2016,"Pagination":"045015","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":264547,"SerRR":"Biomedical Materials. IOP PUBLISHING LTD: Bristol.  ISSN 1748-6041; e-ISSN 1748-605X","StandardTitleSer":"Biomedical Materials","ISSN":"1748-6041","AbbrevSer":null,"StandardTitleMon":null,"StartPage":13,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Douglas","Firstname":"Timothy","Initials":"T.E.L.","Affiliation":"Department of Organic Chemistry, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319198,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Ghent University; Faculty of Sciences; Department of Organic chemistry","ORCID":null,"PersID":35848,"InsID":8466},{"AutName":"Dokupil","Firstname":"Agnieszka","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319199,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Reczynska","Firstname":"Katarzyna","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319200,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Brackman","Firstname":"Gilles","Initials":"G.","Affiliation":"Laboratory of Pharmaceutical Microbiology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":229475,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"LPM","InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Vakgroep Farmaceutische Analyse; Laboratorium voor Farmaceutische Microbiologie","ORCID":null,"PersID":33998,"InsID":12025},{"AutName":"Krok-Borkowicz","Firstname":"Malgorzata","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319202,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Keppler","Firstname":"Julia","Initials":"J.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319203,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bozic","Firstname":"Mojca","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319204,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Van der Voort","Firstname":"Pascal","Initials":"P.","Affiliation":"Department of Inorganic Chemistry, COMOC, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319205,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"COMOC","InsFSN":"Universiteit Gent; Centre for Ordered Materials, Organometallics and Catalysis","ORCID":"0000-0002-1248-479X","PersID":37251,"InsID":14824},{"AutName":"Pietryga","Firstname":"Krzysztof","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319206,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Samal","Firstname":"Sangram","Initials":"S.K.","Affiliation":"Laboratory of General Biochemistry and Physical Pharmacy, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319207,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Laboratorium voor Algemene Biochemie en Fysische Farmacie","ORCID":null,"PersID":null,"InsID":12125},{"AutName":"Balcaen","Firstname":"Lieve","Initials":"L.","Affiliation":"Department of Analytical Chemistry, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":155303,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Chemie","ORCID":"0000-0002-1800-6932","PersID":33987,"InsID":421},{"AutName":"van den Bulcke","Firstname":"Jan","Initials":"J.","Affiliation":"Laboratory of Wood Technology (Woodlab), Department of Forest and Water Management, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319209,"OrderNr":12,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":41124,"InsID":null},{"AutName":"Van Acker","Firstname":"Joris","Initials":"J.","Affiliation":"Laboratory of Wood Technology (Woodlab), Department of Forest and Water Management, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319210,"OrderNr":13,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":22591,"InsID":null},{"AutName":"Vanhaecke","Firstname":"Frank","Initials":"F.","Affiliation":"Department of Analytical Chemistry, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":141337,"OrderNr":14,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Chemie","ORCID":"0000-0002-1884-3853","PersID":31376,"InsID":421},{"AutName":"Schwarz","Firstname":"Karin","Initials":"K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319211,"OrderNr":15,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Coenye","Firstname":"Tom","Initials":"T.","Affiliation":"Laboratory of Pharmaceutical Microbiology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":229476,"OrderNr":16,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"LPM","InsFSN":"Universiteit Gent; Faculteit Farmaceutische Wetenschappen; Vakgroep Farmaceutische Analyse; Laboratorium voor Farmaceutische Microbiologie","ORCID":"0000-0002-6407-0601","PersID":33997,"InsID":12025},{"AutName":"Pamula","Firstname":"Elzbieta","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":285858,"AutID":319213,"OrderNr":17,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":375935,"LibID":36,"BRefID":293794,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"375935","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"IOP PUBLISHING LTD","City":"Bristol"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":285858,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":285858,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1088/1748-6041/11/4/045015","externalID":"10.1088/1748-6041/11/4/045015","URLTypeCode":"DOI","URLID":62686,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2018-03-12 21:25:44.307000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2021-09-08 07:25:08.487000","timezone_type":3,"timezone":"Europe/Brussels"}}}