{"refrec":{"BRefID":294828,"RR":"<b>Bourlat, S.J.; Haenel, Q.; Finnman, J.; Leray, M.</b> (2016). Preparation of amplicon libraries for metabarcoding of marine eukaryotes using Illumina MiSeq: the dual-PCR Method, <b><i>in</i></b>: Bourlat, S.J. (Ed.) <i>Marine genomics: methods and protocols. Methods in Molecular Biology,</i> 1452: pp. 197-207. <a href=\"https://dx.doi.org/10.1007/978-1-4939-3774-5_13\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-4939-3774-5_13</a>","BEntID":286899,"PublicFlag":1,"CheckedFlag":0,"wosflag":null,"vabbflag":null,"RefStringPartII":", <b><i>in</i></b>: Bourlat, S.J. (Ed.) <i>Marine genomics: methods and protocols. Methods in Molecular Biology,</i> 1452: pp. 197-207. <a href=\"https://dx.doi.org/10.1007/978-1-4939-3774-5_13\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-4939-3774-5_13</a>","DocTypID":17,"DocType":"Book chapters","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bourlat, S.J.; Haenel, Q.; Finnman, J.; Leray, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bourlat, S.J. <i>et al.</i>","Englishabstract":"This protocol details the preparation of multiplexed amplicon libraries for metabarcoding (amplicon-based) studies of microscopic marine eukaryotes. Metabarcoding studies, based on the amplification of a taxonomically informative marker from a collection of organisms or an environmental sample, can be performed to analyze biodiversity patterns or predator–prey interactions. For Metazoa, we use the mitochondrial cytochrome oxidase 1 (CO1) or the small ribosomal subunit (SSU) markers. Here, we describe a strategy for the preparation of multiplexed Illumina MiSeq libraries using a dual-PCR approach for the addition of index and adaptor sequences.","AbstractOtherLang":null,"BibLvlCode":"AMS","StandardTitle":"Preparation of amplicon libraries for metabarcoding of marine eukaryotes using Illumina MiSeq: the dual-PCR Method","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Metabarcoding; Biodiversity; Microscopic eukaryotes; Illumina MiSeq ","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2018-04-23","DateCreate":"2018-04-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0,"DOI":"10.1007/978-1-4939-3774-5_13"},"refs":null,"anarec":{"AnaID":294828,"PubliDate":2016,"Pagination":"197-207","XtraPublOfAnaID":null,"ISBN":"978-1-4939-3772-1","Volume":"1452","Issue":null,"BRefMon":294814,"BRefMonRR":"<b>Bourlat, S.J. (Ed.)</b> (2016). Marine genomics: methods and protocols. <i>Methods in Molecular Biology</i>, 1452. Humana Press/Springer Science+Business Media, Inc: New York. ISBN 978-1-4939-3772-1. 253 pp. <a href=\"https://dx.doi.org/10.1007/978-1-4939-3774-5\" target=\"_blank\">https://dx.doi.org/10.1007/978-1-4939-3774-5</a>","BRefXtra":null,"BRefXtraRR":null,"SerBRefID":294813,"SerRR":"Methods in Molecular Biology. Humana Press.  ISSN 1064-3745","StandardTitleSer":"Methods in Molecular Biology","ISSN":"1064-3745","AbbrevSer":null,"StandardTitleMon":"Marine genomics: methods and protocols","StartPage":197,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"MS","SerNotes":null,"AutString":"Bourlat, S.J. (Ed.)"},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Bourlat","Firstname":"Sarah J.","Initials":"S.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":286899,"AutID":322888,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Haenel","Firstname":"Quiterie","Initials":"Q.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":286899,"AutID":322889,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Finnman","Firstname":"Jennie","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":286899,"AutID":322890,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Leray","Firstname":"Matthieu","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":286899,"AutID":322891,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":null,"MONpubls":[{"PublName":"Humana Press","Place":"New York"},{"PublName":"Springer Science+Business Media, Inc","Place":"New York"}],"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":[{"AutName":"Bourlat","Initials":"S.J.","CorporateFlag":0,"BEntID":286885,"AutID":322835,"OrderNr":1,"DegrID":null,"EditorFlag":1,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"AutStrTrunc":"Bourlat, S.J. (Ed.)"}],"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":286899,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":286899,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/978-1-4939-3774-5_13","externalID":"10.1007/978-1-4939-3774-5_13","URLTypeCode":"DOI","URLID":64084,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Verheyde, Fons, F.","newSesDate":{"date":"2018-04-23 09:12:36.600000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Verheyde, Fons, F.","updSesDate":{"date":"2018-04-23 09:12:36.600000","timezone_type":3,"timezone":"Europe/Brussels"}}}