{"refrec":{"BRefID":295455,"RR":"<b>Van Eynde, B.; Christiaens, O.; Delbare, D.; Cooreman, K.; Bateman, K.S.; Stentiford, G.D.; Dullemans, A.M.; van Oers, M.M.; Smagghe, G.</b> (2018). Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp (<i>Crangon crangon</i>). <i>J. Invertebr. Pathol. 153</i>: 195-202. <a href=\"https://dx.doi.org/10.1016/j.jip.2018.03.006\" target=\"_blank\">https://dx.doi.org/10.1016/j.jip.2018.03.006</a>","BEntID":287542,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>J. Invertebr. Pathol. 153</i>: 195-202. <a href=\"https://dx.doi.org/10.1016/j.jip.2018.03.006\" target=\"_blank\">https://dx.doi.org/10.1016/j.jip.2018.03.006</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Van Eynde, B.; Christiaens, O.; Delbare, D.; Cooreman, K.; Bateman, K.S.; Stentiford, G.D.; Dullemans, A.M.; van Oers, M.M.; Smagghe, G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Van Eynde, B. <i>et al.</i>","Englishabstract":"<i>Crangon crangon</i> bacilliform virus (CcBV) was first discovered in 2004 in European brown shrimp (<i>Crangon crangon</i>) caught along the English coast. This study describes a duplex PCR assay developed for the detection of CcBV, based on amplification of the lef-8 gene (211 bp) of CcBV and the E75 gene (105 bp) of <i>C. crangon</i> as an internal amplification control. The lef-8 and E75 primer pairs were designed based on preliminary genome sequencing information of the virus and transcriptomic data available for <i>C. crangon</i>, respectively. Sequencing of the resulting amplicons confirmed the specificity of this PCR assay and sequence analysis of the lef-8 fragment revealed amino acid identity percentages ranging between 31 and 42% with members of the Nudiviridae, proposing that CcBV may reside within this family.Finally, the duplex PCR assay was applied to samples of <i>C. crangon</i>hepatopancreas tissue collected along the Belgian coast to screen for the presence of CcBV. The prevalence of CcBV averaged 87%, which is comparable to previous reports of high prevalence, based upon histological analysis, in shrimp collected along the English coast. Development of a specific and sensitive PCR assay to detect CcBV will provide a useful tool for future aquaculture and research programs involving <i>C. crangon</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Development and application of a duplex PCR assay for detection of Crangon crangon bacilliform virus in populations of European brown shrimp (<i>Crangon crangon</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:32:40.612146","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Crustacean; Disease; CcBV; Nudiviridae; Diagnostic; Aquaculture","OtherDescriptors":null,"Notes":null,"AnaPub":2018,"MonPub":null,"DateUpdate":"2020-05-20","DateCreate":"2018-05-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000429892400026","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.jip.2018.03.006"},"refs":null,"anarec":{"AnaID":295455,"PubliDate":2018,"Pagination":"195-202","XtraPublOfAnaID":null,"ISBN":null,"Volume":"153","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43073,"SerRR":"Journal of Invertebrate Pathology. Academic Press: New York.  ISSN 0022-2011; e-ISSN 1096-0805","StandardTitleSer":"Journal of Invertebrate Pathology","ISSN":"0022-2011","AbbrevSer":"J. Invertebr. Pathol.","StandardTitleMon":null,"StartPage":195,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Van Eynde","Firstname":"Benigna","Initials":"B.","Affiliation":"Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":325220,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Ghent University; Department of crop protection","ORCID":null,"PersID":31886,"InsID":8795},{"AutName":"Christiaens","Firstname":"Olivier","Initials":"O.","Affiliation":"Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":325221,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Ghent University; Department of crop protection","ORCID":"0000-0001-5850-340X","PersID":34096,"InsID":8795},{"AutName":"Delbare","Firstname":"Daan","Initials":"D.","Affiliation":"Animal Sciences Unit, Fisheries, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO)","Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":325222,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw- , visserij en voedingsonderzoek; Kenniseenheid: Dier; Visserij & Aquatische Productie","ORCID":"0000-0002-4220-5421","PersID":124,"InsID":12746},{"AutName":"Cooreman","Firstname":"Kris","Initials":"K.","Affiliation":"Animal Sciences Unit, Fisheries, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO)","Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":325223,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw- , visserij en voedingsonderzoek; Kenniseenheid: Dier; Visserij & Aquatische Productie","ORCID":null,"PersID":350,"InsID":12746},{"AutName":"Bateman","Firstname":"Kelly","Initials":"K.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":325224,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Stentiford","Firstname":"Grant","Initials":"G.D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":15158,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dullemans","Firstname":"Annette","Initials":"A.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":325226,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"van Oers","Firstname":"Monique","Initials":"M.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":325227,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Smagghe","Firstname":"Guy","Initials":"G.","Affiliation":"Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":287542,"AutID":142759,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"FBW","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen","ORCID":null,"PersID":16121,"InsID":7348}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":333187,"LibID":36,"BRefID":295455,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"333187","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Academic Press","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Crustacea"}],"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"720":{"SpName":"Simon Stevin acknowledged","SpColID":720,"ParSpColID":39,"TopParID":39,"ShortName":"Simon Stevin","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/Simon Stevin"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":287542,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":287542,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.jip.2018.03.006","externalID":"10.1016/j.jip.2018.03.006","URLTypeCode":"DOI","URLID":64507,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Crustacea [crustaceans]","AphiaID":1066,"TaxtID":15633}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Crustacea","DutchTerm":"Kreeftachtigen","AphiaID":1066,"TaxtID":15633}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Lyssens, Liesbeth, L.","newSesDate":{"date":"2018-05-15 12:32:46.633000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lust, Heike, H.","updSesDate":{"date":"2020-05-20 09:39:20.887000","timezone_type":3,"timezone":"Europe/Brussels"}}}