{"refrec":{"BRefID":297758,"RR":"<b>Sorokin, D.Y.; Merkel, A.Y.; Abbas, B.; Makarova, K.S.; Rijpstra, W.I.C.; Koenen, M.; Sinninghe Damsté, J.S.; Galinski, E.A.; Koonin, E.V.; van Loosdrecht, M.C.M.</b> (2018). <i>Methanonatronarchaeum thermophilum</i> gen. nov., sp. nov. and '<i>Candidatus</i> Methanohalarchaeum thermophilum', extremely halo(natrono)philic methyl-reducing methanogens from hypersaline lakes comprising a new euryarchaeal class <i>Methanonatronarchaeia</i> classis nov. <i>Int. J. Syst. Evol. Microbiol. 68(7)</i>: 2199-2208. <a href=\"https://doi.org/10.1099/ijsem.0.002810\" target=\"_blank\">https://doi.org/10.1099/ijsem.0.002810</a>","BEntID":289846,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":" <i>Int. J. Syst. Evol. Microbiol. 68(7)</i>: 2199-2208. <a href=\"https://doi.org/10.1099/ijsem.0.002810\" target=\"_blank\">https://doi.org/10.1099/ijsem.0.002810</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sorokin, D.Y.; Merkel, A.Y.; Abbas, B.; Makarova, K.S.; Rijpstra, W.I.C.; Koenen, M.; Sinninghe Damsté, J.S.; Galinski, E.A.; Koonin, E.V.; van Loosdrecht, M.C.M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sorokin, D.Y. <i>et al.</i>","Englishabstract":"Methanogenic enrichments from hypersaline lakes at moderate thermophilic conditions have resulted in the cultivation of an unknown deep lineage of euryarchaeota related to the class <i>Halobacteria</i>. Eleven soda lake isolates and three salt lake enrichment cultures were methyl-reducing methanogens that utilize C>sub>1</sub>methylated compounds as electron acceptors and H<sub>2</sub>or formate as electron donors, but they were unable to grow on either substrates alone or to form methane from acetate. They are extreme halophiles, growing optimally at 4 M total Na<super>+</super> and the first representatives of methanogens employing the ‘salt-in’ osmoprotective mechanism. The salt lake subgroup is neutrophilic, whereas the soda lake isolates are obligate alkaliphiles, with an optimum around pH 9.5. Both grow optimally at 50 °C. The genetic diversity inside the two subgroups is very low, indicating that the soda and salt lake clusters consist of a single genetic species each. The phylogenetic distance between the two subgroups is in the range of distant genera, whereas the distance to other euryarchaea is below 83 % identity of the 16S rRNA gene. These isolates and enriched methanogens, together with closely related environmental clones from hypersaline habitats (the SA1 group), form a novel class-level clade in the phylum Euryarchaeota. On the basis of distinct phenotypic and genetic properties, the soda lake isolates are classified into a new genus and species, <i>Methanonatronarchaeum thermophilum</i>, with the type strain AMET1<super>T</super> (DSM 28684<super>T</super> =NBRC 110805<super>T</super>= UNIQEM U982<super>T</super>), and the salt lake methanogens into a candidate genus and species ‘<i>Candidatus</i> Methanohalarchaeum thermophilum’. These organisms are proposed to form novel family, order and class <i>Methanonatronarchaeaceae</i>fam","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"<i>Methanonatronarchaeum thermophilum</i> gen. nov., sp. nov. and '<i>Candidatus</i> Methanohalarchaeum thermophilum', extremely halo(natrono)philic methyl-reducing methanogens from hypersaline lakes comprising a new euryarchaeal class <i>Methanonatronarchaeia</i> classis nov.","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"hypersaline; soda lakes; methanogenesis; methyl-reducing pathway; Methanonatronarchaeia","OtherDescriptors":null,"Notes":null,"AnaPub":2018,"MonPub":null,"DateUpdate":"2019-03-21","DateCreate":"2018-07-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000437232500013","VABBcode":null,"OpenAcc":0,"DOI":"10.1099/ijsem.0.002810"},"refs":null,"anarec":{"AnaID":297758,"PubliDate":2018,"Pagination":"2199-2208","XtraPublOfAnaID":null,"ISBN":null,"Volume":"68","Issue":"7","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":56795,"SerRR":"International Journal of Systematic and Evolutionary Microbiology. 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