{"refrec":{"BRefID":297784,"RR":"<b>Koulman, A.; Pruijn, L.M.C.; Sandstra, T.S.A.; Woerdenbag, H.J.; Pras, N.</b> (1999). The pharmaceutical exploration of cold water ascidians from the Netherlands: a possible source of new cytotoxic natural products. <i>Journal of biotechnology 70(1-3)</i>: 85-88. <a href=\"https://dx.doi.org/10.1016/s0168-1656(99)00062-0\" target=\"_blank\">https://dx.doi.org/10.1016/s0168-1656(99)00062-0</a>","BEntID":289872,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Journal of biotechnology 70(1-3)</i>: 85-88. <a href=\"https://dx.doi.org/10.1016/s0168-1656(99)00062-0\" target=\"_blank\">https://dx.doi.org/10.1016/s0168-1656(99)00062-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Koulman, A.; Pruijn, L.M.C.; Sandstra, T.S.A.; Woerdenbag, H.J.; Pras, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Koulman, A. <i>et al.</i>","Englishabstract":"Six ascidian species from the Dutch North Sea coast were screened for cytotoxic activity. Freeze-dried biological material was extracted with solvents of different polarity followed by determination of the cytotoxicity. The most active extracts were further separated using different chromatographic techniques. The microculture tetrazolium (MTT) assay was used to determine the cytotoxicity against two human tumor cell lines: COLO<sub>320</sub> (a colon adenocarcinoma) and GLC<sub>4</sub> (a small cell lung carcinoma). The two cell lines were selected for their different response towards known cytostatics. GLC<sub>4</sub> is sensitive and COLO<sub>320</sub> is resistant to most of the known cytostatics. Three of the species tested yielded interesting fractions. From the in colonial <em>Didemnum lahillei</em> we have isolated a compound which is more active against the COLO<sub>320</sub> cell line (IC<sub>50</sub>:33 μg ml<sup>−1</sup>) than the GLC<sub>4</sub> (IC<sub>50</sub>:49 μg ml<sup>−1</sup>). The structure is currently elucidated. Another colonial species, <em>Aplidium glabrum</em>, yielded a very cytotoxic fraction, with an IC<sub>50</sub> of 5 μg ml<sup>−1</sup> against the COLO<sub>320</sub> cell line. From our results it can be concluded that the North Sea could be another interesting source of new compounds with pharmaceutical potential.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The pharmaceutical exploration of cold water ascidians from the Netherlands: a possible source of new cytotoxic natural products","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:33:14.980000","timezone_type":1,"timezone":"+00:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Microculture tetrazolium assay; Ascidian","OtherDescriptors":null,"Notes":null,"AnaPub":1999,"MonPub":null,"DateUpdate":"2018-07-05","DateCreate":"2018-07-05","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000081185800012","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/s0168-1656(99)00062-0"},"refs":null,"anarec":{"AnaID":297784,"PubliDate":1999,"Pagination":"85-88","XtraPublOfAnaID":null,"ISBN":null,"Volume":"70","Issue":"1-3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":185369,"SerRR":"Journal of biotechnology. Elsevier.  ISSN 0168-1656; e-ISSN 1873-4863","StandardTitleSer":"Journal of biotechnology","ISSN":"0168-1656","AbbrevSer":null,"StandardTitleMon":null,"StartPage":85,"Pages":4,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Koulman","Firstname":"Albert","Initials":"A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":289872,"AutID":36667,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Pruijn","Firstname":"Loes","Initials":"L.M.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":289872,"AutID":335745,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sandstra","Firstname":"Thérèse","Initials":"T.S.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":289872,"AutID":335746,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Woerdenbag","Firstname":"Herman","Initials":"H.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":289872,"AutID":335747,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Pras","Firstname":"Niesko","Initials":"N.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":289872,"AutID":335748,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":315184,"LibID":36,"BRefID":297784,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"315184","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":null}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"ANE, North Sea","ThestID":182108,"Acronym":"ASFA_GEOT","ThesTermPath":"ANE, North Sea"},{"ThesaurusTerm":"Cytotoxicity","ThestID":9496,"Acronym":"ASFA","ThesTermPath":"Properties > Biological properties > Toxicity > Cytotoxicity"}],"thestermsASFA":[{"ThesaurusTerm":"ANE, North Sea"},{"ThesaurusTerm":"Cytotoxicity"}],"taxtermsASFA":[{"TaxTerm":"Aplidium glabrum"},{"TaxTerm":"Ascidiacea"},{"TaxTerm":"Didemnum lahillei"}],"geotermsASFA":[{"GeoTerm":"North Sea"}],"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":289872,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":289872,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/s0168-1656(99)00062-0","externalID":"10.1016/s0168-1656(99)00062-0","URLTypeCode":"DOI","URLID":65971,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"ANE, North Sea","ThestID":182108,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Cytotoxicity","ThestID":9496,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Aplidium glabrum","AphiaID":103647,"TaxtID":44327},{"TaxTerm":"Ascidiacea [sea squirts]","AphiaID":1839,"TaxtID":19381},{"TaxTerm":"Didemnum lahillei","AphiaID":103569,"TaxtID":44326}],"geoterms":[{"GeoTerm":"North Sea","GeoObjectID":2350,"GeotID":390}],"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"ANE, North Sea","DutchTerm":null,"ThestID":182108,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Cytotoxicity","DutchTerm":null,"ThestID":9496,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Aplidium glabrum","DutchTerm":null,"AphiaID":103647,"TaxtID":44327},{"TaxTerm":"Ascidiacea","DutchTerm":"Zakpijpen","AphiaID":1839,"TaxtID":19381},{"TaxTerm":"Didemnum lahillei","DutchTerm":null,"AphiaID":103569,"TaxtID":44326}],"geotermsFRIS":[{"GeoTerm":"North Sea","DutchTerm":"Noordzee"}],"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2018-07-05 08:26:56.773000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2018-07-05 08:26:56.773000","timezone_type":3,"timezone":"Europe/Brussels"}}}