{"refrec":{"BRefID":2979,"RR":"<b>Tzovenis, I.; De Pauw, N.; Sorgeloos, P.</b> (1997). Effect of different light regimes on the docosahexaenoic acid (DHA) content of <i>Isochrysis</i> aff. <i>galbana</i> (clone T-ISO). <i>Aquacult. Int. 5</i>: 489-507. <a href=\"https://dx.doi.org/10.1023/A:1018349131522\" target=\"_blank\">https://dx.doi.org/10.1023/A:1018349131522</a>","BEntID":2979,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Aquacult. Int. 5</i>: 489-507. <a href=\"https://dx.doi.org/10.1023/A:1018349131522\" target=\"_blank\">https://dx.doi.org/10.1023/A:1018349131522</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tzovenis, I.; De Pauw, N.; Sorgeloos, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tzovenis, I.; De Pauw, N.; Sorgeloos, P.","Englishabstract":"The culture of <i>Isochrysis</i> aff. <i>galbana</i> Green (T-ISO) in hatcheries is promoted for its high docosahexaenoic acid (DHA) content which favours the successful rearing of aquatic larvae. In this paper we identified the need for well-defined culture conditions of microalgae if sufficient DHA content is the final goal. Using photoperiods of 24:0h, 12:12h and 16:8h L/D combined with photon flux densities (PFD's) of 110, 220 and 460 µE m<sup>-2</sup> s<sup>-1</sup>, batch cultures of T-ISO were sampled for DHA content in the exponential as well as the post-exponential (light-limited) growth phase. The various light regimes resulted in differences of DHA content of T-ISO revealing influences and strong interactions among the three variables studied. For fast and economic production of T-ISO cells rich in DHA it is recommended to harvest batch cultures at the end of the exponential phase using high photon flux densities (PFD's) under 12:12 h L/D cycles. The same regime or lower PFD under 24:0 h L/D seem to be sufficient for a continuous culture optimization scheme. In any case a strict following of culture protocols is very important since there is evidence for coexistence of different clones within the parental cultures leading to different results depending on which one prevails.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effect of different light regimes on the docosahexaenoic acid (DHA) content of <i>Isochrysis</i> aff. <i>galbana</i> (clone T-ISO)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:31:15.971977","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1997,"MonPub":null,"DateUpdate":"2000-10-19","DateCreate":"2000-08-28","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1997YK12000002","VABBcode":null,"OpenAcc":0,"DOI":"10.1023/A:1018349131522"},"refs":null,"anarec":{"AnaID":2979,"PubliDate":1997,"Pagination":"489-507","XtraPublOfAnaID":null,"ISBN":null,"Volume":"5","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44242,"SerRR":"Aquaculture International. 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