{"refrec":{"BRefID":299094,"RR":"<b>Claereboudt, E.J.S.; Eeckhaut, I.; Lins, L.; Deleu, M.</b> (2018). How different sterols contribute to saponin tolerant plasma membranes in sea cucumbers. <i>NPG Scientific Reports 8(1)</i>: 11 pp. <a href=\"https://dx.doi.org/10.1038/s41598-018-29223-x\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-018-29223-x</a>","BEntID":291298,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 8(1)</i>: 11 pp. <a href=\"https://dx.doi.org/10.1038/s41598-018-29223-x\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-018-29223-x</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Claereboudt, E.J.S.; Eeckhaut, I.; Lins, L.; Deleu, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Claereboudt, E.J.S. <i>et al.</i>","Englishabstract":"Sea cucumbers produce saponins as a chemical defense mechanism, however their cells can tolerate the cytotoxic nature of these chemicals. To elucidate the molecular mechanisms behind this tolerance a suite of complementary biophysical tools was used, firstly using liposomes for <i>in vitro</i> techniques then using <i>in silico</i> approaches for a molecular-level insight. The holothuroid saponin Frondoside A, caused significantly less permeabilization in liposomes containing a Δ<sup>7</sup> holothuroid sterol than those containing cholesterol and resulted in endothermic interactions versus exothermic interactions with cholesterol containing liposomes. Lipid phases simulations revealed that Frondoside A has an agglomerating effect on cholesterol domains, however, induced small irregular Δ<sup>7</sup> sterol clusters. Our results suggest that the structural peculiarities of holothuroid sterols provide sea cucumbers with a mechanism to mitigate the sterol-agglomerating effect of saponins, and therefore to protect their cells from the cytotoxicity of the saponins they produce.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"How different sterols contribute to saponin tolerant plasma membranes in sea cucumbers","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:31:55.781580","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2018,"MonPub":null,"DateUpdate":"2018-07-23","DateCreate":"2018-07-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000439026000006","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-018-29223-x"},"refs":null,"anarec":{"AnaID":299094,"PubliDate":2018,"Pagination":"11 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"8","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Claereboudt","Firstname":"Emily","Initials":"E.J.S.","Affiliation":"Biology of Marine Organisms and Biomimetics, Research Institute for Biosciences, University of Mons","Discriminator":null,"CorporateFlag":0,"BEntID":291298,"AutID":337048,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"BOMB","InsFSN":"Université de Mons; Faculté des Sciences; Département de Biologie; Laboratoire de Biologie des Organismes Marins et Biomimétisme","ORCID":"0000-0003-2336-1960","PersID":34142,"InsID":12895},{"AutName":"Eeckhaut","Firstname":"Igor","Initials":"I.","Affiliation":"Biology of Marine Organisms and Biomimetics, Research Institute for Biosciences, University of Mons","Discriminator":null,"CorporateFlag":0,"BEntID":291298,"AutID":337050,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"BOMB","InsFSN":"Université de Mons; Faculté des Sciences; Département de Biologie; Laboratoire de Biologie des Organismes Marins et Biomimétisme","ORCID":null,"PersID":2317,"InsID":12895},{"AutName":"Lins","Firstname":"Laurence","Initials":"L.","Affiliation":"Laboratory of molecular biophysics of interfaces, Gembloux Agro-Bio Tech, University of Liege","Discriminator":null,"CorporateFlag":0,"BEntID":291298,"AutID":337051,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Gembloux Agro-Bio Tech","ORCID":null,"PersID":null,"InsID":579},{"AutName":"Deleu","Firstname":"Magali","Initials":"M.","Affiliation":"Laboratory of molecular biophysics of interfaces, Gembloux Agro-Bio Tech, University of Liege","Discriminator":null,"CorporateFlag":0,"BEntID":291298,"AutID":337052,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Université de Liège; Gembloux Agro-Bio Tech","ORCID":null,"PersID":null,"InsID":579}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":316484,"LibID":36,"BRefID":299094,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"316484","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":291298,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":291298,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-018-29223-x","externalID":"10.1038/s41598-018-29223-x","URLTypeCode":"DOI","URLID":66155,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2018-07-23 08:57:32.283000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2018-07-23 08:57:32.283000","timezone_type":3,"timezone":"Europe/Brussels"}}}