{"refrec":{"BRefID":300371,"RR":"<b>Declercq, A.M.; Chiers, K.; Van den Broeck, W.; Dewulf, J.; Eeckhaut, V.; Cornelissen, M.; Bossier, P.; Haesebrouck, F.; Decostere, A.</b> (2015). Interactions of highly and low virulent <i>Flavobacterium columnare</i> isolates with gill tissue in carp and rainbow trout. <i>Vet. Res. 46(1)</i>: 25. <a href=\"https://dx.doi.org/10.1186/s13567-015-0164-5\" target=\"_blank\">https://dx.doi.org/10.1186/s13567-015-0164-5</a>","BEntID":292607,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Vet. Res. 46(1)</i>: 25. <a href=\"https://dx.doi.org/10.1186/s13567-015-0164-5\" target=\"_blank\">https://dx.doi.org/10.1186/s13567-015-0164-5</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":1,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Declercq, A.M.; Chiers, K.; Van den Broeck, W.; Dewulf, J.; Eeckhaut, V.; Cornelissen, M.; Bossier, P.; Haesebrouck, F.; Decostere, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Declercq, A.M. <i>et al.</i>","Englishabstract":"The interactions of <i>Flavobacterium columnare</i> isolates of different virulence with the gills of carp (<i>Cyprinus carpio</i> L.) and rainbow trout (<i>Oncorhynchus mykiss</i> Walbaum) were investigated. Both fish species were exposed to different high (HV) or low virulence (LV) isolates and sacrificed at seven predetermined times post-challenge. Histopathological and ultrastructural examination of carp and rainbow trout inoculated with the HV-isolate disclosed bacterial invasion and concomitant destruction of the gill tissue, gradually spreading from the filament tips towards the base, with outer membrane vesicles surrounding most bacterial cells. In carp, 5-10% of the fish inoculated with the LV-isolate became moribund and their gill tissue displayed the same features as described for the HV-isolate, albeit to a lesser degree. The bacterial numbers retrieved from the gill tissue were significantly higher for HV- compared to LV-isolate challenged carp and rainbow trout. TUNEL-stained and caspase-3-immunostained gill sections demonstrated significantly higher apoptotic cell counts in carp and rainbow trout challenged with the HV-isolate compared to control animals. Periodic acid-<i>Schiff</i>/alcian blue staining demonstrated a significantly higher total gill goblet cell count for HV- and LV-isolate challenged compared to control carp. Moreover, bacterial clusters were embedded in a neutral matrix while being encased by acid mucins, resembling biofilm formation. Eosinophilic granular cell counts were significantly higher in the HV-isolate compared to LV-isolate inoculated and control carp. The present data indicate a high colonization capacity, and the destructive and apoptotic-promoting features of the HV-isolate, and point towards important dynamic host mucin–<i>F. columnare</i> interactions warranting further research.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Interactions of highly and low virulent <i>Flavobacterium columnare</i> isolates with gill tissue in carp and rainbow trout","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:32:02.354747","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Rainbow Trout; Goblet Cell; Highly Virulent; Gill Tissue; Gill Filament","OtherDescriptors":null,"Notes":null,"AnaPub":2015,"MonPub":null,"DateUpdate":"2018-08-23","DateCreate":"2018-08-23","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000350376700001","VABBcode":null,"OpenAcc":1,"DOI":"10.1186/s13567-015-0164-5"},"refs":null,"anarec":{"AnaID":300371,"PubliDate":2015,"Pagination":"25","XtraPublOfAnaID":null,"ISBN":null,"Volume":"46","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44064,"SerRR":"Veterinary Research. 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