Document of bibliographic reference 300637
BibliographicReference record
- Type
- Bibliographic resource
- Type of document
- Journal article
- BibLvlCode
- AS
- Title
- Scanning electron microscopic aids for identification of larval and post-larval bivalves
- Abstract
- The identification of bivalve larvae and early postlarvae in plankton and benthic samples has long been a challenge, hampering both basic and applied research efforts in marine, estuarine, and freshwater environments. The usefulness of published optical micrographs of the early life-history stages of bivalves is limited because of the great morphological similarity of the imaged articulated shells, particularly at the early (straight-hinge) developmental stages. While a number of techniques have been refined in recent years and show promise for use in routine identifications of larval and post-larval bivalves (e.g., single-step nested multiplex polymerase chain reaction; in situ hybridization protocols through color coding with taxon-specific, dye-labeled DNA probes; coupled fluorescence in situ hybridization and cell sorting; and image analysis techniques using species-specific shell birefringence patterns under polarized light), no adequate comprehensive reference source exists that accurately depicts the morphology and morphometry of the shells of larval and post-larval stages of target bivalve species in a consistent format to assist in identification of such stages. To this end, scanning electron micrograph (SEM) sequences are presented of the disarticulated shell valves of laboratory-reared larval and post-larval stages of 56 species of bivalve molluscs from a wide spectrum of marine, estuarine, and freshwater habitats. Emphasis is placed on the usefulness of the morphology and morphometrics of consistently-oriented, disarticulated shell valves and associated hinge structures in discriminating the early life-history stages of these various bivalve species. Although the scanning electron micrograph sequences presented accurately depict the gross morphologies/ morphometrics and hinge structures of the disarticulated shell valves of the larvae and/or postlarvae of the 56 species of bivalves, it is important to emphasize that a scanning electron microscope is not necessary to observe even fine hinge structures associated with the early ontogenetic stages of these species. Such structures are readily visible using a wide range of optical compound microscopes equipped with high-intensity reflected light sources, although the disarticulated shell valves must be viewed in several planes of focus to discern the often subtle details seen clearly in the scanning electron micrographs. These morphological characters provide researchers with invaluable aids for the routine identification of the early life-history stages of these species isolated from plankton and benthic samples.
- WebOfScience code
- https://www.webofscience.com/wos/woscc/full-record/WOS:000435219000002
- Bibliographic citation
- Lutz, R.A.; Goodwin, J.D.; Baldwin, B.S.; Burnell, G.; Castagna, M.; Chapman, S.; Chestnut, A.; Dabinett, P.; Davis, C.; Eversole, A.G.; Fuller, S.C.; Gallager, S.M.; Goldberg, R.; Goodsell, J.; Grassle, J.; Gustafson, R.G.; Hidu, H.; Hu, Y.-P.; Jablonski, D.; Johnson, S.; Kennedy, V.S.; Pennec, M.L.; Mann, R.; Newell, C.; Pooley, A.S.; Tan, A.S.; Vrijenhoek, R.C.; Partridge, A. (2018). Scanning electron microscopic aids for identification of larval and post-larval bivalves. J. Shellfish Res. 37(2): 247-448. https://dx.doi.org/10.2983/035.037.0202
- Topic
- Marine
- Fresh water
- Brakish water
- Is peer reviewed
- true
Authors
- author
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- Name
- Richard Lutz
- author
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- Name
- Jacob Goodwin
- author
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- Name
- Brad Baldwin
- author
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- Name
- Gavin Burnell
- author
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- Name
- Michael Castagna
- author
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- Name
- Samuel Chapman
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- Name
- Al Chestnut
- author
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- Name
- Patrick Dabinett
- author
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- Name
- Chris Davis
- author
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- Name
- Arnold Eversole
- author
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- Name
- S. Cynthia Fuller
- author
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- Name
- Scott Gallager
- author
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- Name
- Ronald Goldberg
- author
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- Name
- Joy Goodsell
- author
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- Name
- Judith Grassle
- author
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- Name
- Richard Gustafson
- author
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- Name
- Herbert Hidu
- author
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- Name
- Ya-Ping Hu
- author
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- Name
- David Jablonski
- author
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- Name
- Shannon Johnson
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- Name
- Victor Kennedy
- author
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- Name
- Marcel Le Pennec
- author
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- Name
- Roger Mann
- author
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- Name
- Carter Newell
- author
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- Name
- Alan Pooley
- author
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- Name
- Antonieto Tan
- author
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- Name
- Robert Vrijenhoek
- author
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taxonomic terms
- taxonomic terms associated with this publication
- Bivalvia