{"refrec":{"BRefID":301312,"RR":"<b>Brackman, G.; Celen, S.; Baruah, K.; Bossier, P.; Van Calenbergh, S.; Nelis, H.J.; Coenye, T.</b> (2009). Al-2 quorum-sensing inhibitors affect the starvation response and reduce virulence in several <i>Vibrio</i> species, most likely by interfering with LuxPQ. <i>Microbiology 155</i>: 4114-4122. <a href=\"https://dx.doi.org/10.1099/mic.0.032474-0\" target=\"_blank\">https://dx.doi.org/10.1099/mic.0.032474-0</a>","BEntID":293552,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Microbiology 155</i>: 4114-4122. <a href=\"https://dx.doi.org/10.1099/mic.0.032474-0\" target=\"_blank\">https://dx.doi.org/10.1099/mic.0.032474-0</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Brackman, G.; Celen, S.; Baruah, K.; Bossier, P.; Van Calenbergh, S.; Nelis, H.J.; Coenye, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Brackman, G. <i>et al.</i>","Englishabstract":"The increase of disease outbreaks caused by <i>Vibrio</i> species in aquatic organisms as well as in humans, together with the emergence of antibiotic resistance in <i>Vibrio</i> species, has led to a growing interest in alternative disease control measures. Quorum sensing (QS) is a mechanism for regulating microbial gene expression in a cell density-dependent way. While there is good evidence for the involvement of auto-inducer 2 (AI-2)-based interspecies QS in the control of virulence in multiple <i>Vibrio</i> species, only few inhibitors of this system are known. From the screening of a small panel of nucleoside analogues for their ability to disturb AI-2-based QS, an adenosine derivative with a <i>p</i>-methoxyphenylpropionamide moiety at C-3′ emerged as a promising hit. Its mechanism of inhibition was elucidated by measuring the effect on bioluminescence in a series of <i>Vibrio harveyi</i> AI-2 QS mutants. Our results indicate that this compound, as well as a truncated analogue lacking the adenine base, block AI-2-based QS without interfering with bacterial growth. The active compounds affected neither the bioluminescence system as such nor the production of AI-2, but most likely interfered with the signal transduction pathway at the level of LuxPQ in <i>V. harveyi</i>. The most active nucleoside analogue (designated LMC-21) was found to reduce the <i>Vibrio</i> species starvation response, to affect biofilm formation in <i>Vibrio anguillarum</i>, <i>Vibrio vulnificus</i> and <i>Vibrio cholerae</i>, to reduce pigment and protease production in <i>V. anguillarum</i>, and to protect gnotobiotic <i>Artemia</i> from <i>V. harveyi</i>-induced mortality.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Al-2 quorum-sensing inhibitors affect the starvation response and reduce virulence in several <i>Vibrio</i> species, most likely by interfering with LuxPQ","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2009,"MonPub":null,"DateUpdate":"2018-09-21","DateCreate":"2018-09-14","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000272918200031","VABBcode":null,"OpenAcc":1,"DOI":"10.1099/mic.0.032474-0"},"refs":null,"anarec":{"AnaID":301312,"PubliDate":2009,"Pagination":"4114-4122","XtraPublOfAnaID":null,"ISBN":null,"Volume":"155","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43402,"SerRR":"Microbiology. 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