{"refrec":{"BRefID":301778,"RR":"<b>Kong, J.; Liu, C.; Wang, T.; Yang, D.; Yan, Y.; Chen, Y.; Liu, Y.; Huang, J.; Zheng, G.; Xie, L.; Zhang, R.</b> (2018). Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of <i>Pinctada fucata</i>. <i>NPG Scientific Reports 8(1)</i>: 13 pp. <a href=\"https://dx.doi.org/10.1038/s41598-018-29743-6\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-018-29743-6</a>","BEntID":294019,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 8(1)</i>: 13 pp. <a href=\"https://dx.doi.org/10.1038/s41598-018-29743-6\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-018-29743-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kong, J.; Liu, C.; Wang, T.; Yang, D.; Yan, Y.; Chen, Y.; Liu, Y.; Huang, J.; Zheng, G.; Xie, L.; Zhang, R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kong, J. <i>et al.</i>","Englishabstract":"Shell matrix proteins (SMPs) have important functions in biomineralization. In the past decades, the roles of SMPs were gradually revealed. In 2015, our group identified 72 unique SMPs in <i>Pinctada fucata</i>, among which Alveoline-like (Alv) protein was reported to have homologous genes in <i>Pinctada maxima</i> and <i>Pinctada margaritifera</i>. In this study, the full-length cDNA sequence of <i>Alv</i> and the functional analysis of Alv protein during shell formation were explored. The deduced protein (Alv), which has a molecular mass of 24.9 kDa and an isoelectric point of 11.34, was characterized, and the functional analyses was explored <i>in vivo</i> and <i>in vitro</i>. The <i>Alv</i> gene has high expression in mantle and could response to notching damage. The functional inhibition of Alv protein <i>in vivo</i> by injecting recombinant Alv (rAlv) antibodies destroyed prism structure but accelerated nacre growth. Western blot and immunofluorescence staining showed that native Alv exists in the EDTA-insoluble matrix of both prismatic and nacreous layers and has different distribution patterns in the inner or outer prismatic layer. Taken together, the characterization and functional analyses of matrix protein Alv could expand our understanding of basic matrix proteins and their functions during shell formation.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of <i>Pinctada fucata</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-17 01:33:37.513115","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2018,"MonPub":null,"DateUpdate":"2018-10-03","DateCreate":"2018-10-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000441775900022","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-018-29743-6"},"refs":null,"anarec":{"AnaID":301778,"PubliDate":2018,"Pagination":"13 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"8","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Kong","Firstname":"Jingjing","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":203936,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Liu","Firstname":"Chuang","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":201476,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wang","Firstname":"Tianpeng","Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":203937,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Yang","Firstname":"Dong","Initials":"D.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":57605,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Yan","Firstname":"Yi","Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":346038,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chen","Firstname":"Yan","Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":173646,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Liu","Firstname":"Yangjia","Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":201479,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Huang","Firstname":"Jingliang","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":201478,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Zheng","Firstname":"Guilan","Initials":"G.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":346039,"OrderNr":9,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Xie","Firstname":"Liping","Initials":"L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":50872,"OrderNr":10,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Zhang","Firstname":"Rongqing","Initials":"R.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":294019,"AutID":50873,"OrderNr":11,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":319649,"LibID":36,"BRefID":301778,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"319649","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":294019,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":294019,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-018-29743-6","externalID":"10.1038/s41598-018-29743-6","URLTypeCode":"DOI","URLID":68127,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2018-10-03 08:39:40.900000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2018-10-03 08:39:40.900000","timezone_type":3,"timezone":"Europe/Brussels"}}}