{"refrec":{"BRefID":302936,"RR":"<b>Eamsobhana, P.; Yong, H.S.; Song, S.L.; Tungtrongchitr, A.; Roongruangchai, K.</b> (2018). Genetic differentiation of <i>Anisakis</i> species (Nematoda: Anisakidae) in marine fish <i>Priacanthus tayenus</i> from Gulf of Thailand. <i>Tropical Biomedicine 35(3)</i>: 669-677","BEntID":295184,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Tropical Biomedicine 35(3)</i>: 669-677","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Eamsobhana, P.; Yong, H.S.; Song, S.L.; Tungtrongchitr, A.; Roongruangchai, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Eamsobhana, P. <i>et al.</i>","Englishabstract":"Members of the genus <i>Anisakis</i> are parasitic nematodes of the family Anisakidae. They are potential zoonotic parasites, causing anisakiasis in humans who consume raw or undercooked seafood (fish or squid) infected with the larvae of this nematode. In the present study, anisakid nematodes collected from the marine fish <i>Priacanthus tayenus</i> (Purplespotted big-eye) caught from the Gulf of Thailand were examined morphologically and characterized genetically by DNA sequence analysis. Sequence data from the mitochondrial cytochrome c oxidase subunit II (mtDNA <i>cox2</i>) gene were used to identify these nematodes to species level and to evaluate the phylogenetic relationship among various taxa. All the 15 third-stage larvae of <i>Anisakis</i> nematodes investigated in this study belonged to the same genetic lineage as the <i>A. typica</i> species complex (named here as <i>A. typica</i> sp. T – T for Thailand). Eight mtDNA <i>cox2</i> haplotypes were revealed in the 15 isolates of this <i>Anisakis</i> from Thailand. The mtDNA <i>cox2</i> haplotypes of <i>A. typica</i> sp. T from Thailand were genetically distinct from those of the A. typica sensu stricto. Taxonomic description of this <i>A. typica</i> sp. 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