{"refrec":{"BRefID":303956,"RR":"<b>Qu, M.; Ding, J.; Wang, Y.; Chen, S.; Zhang, Y.; Di, Y.</b> (2019). Genetic impacts induced by BaP and Pb in <i>Mytilus coruscus</i>: Can RAPD be a validated tool in genotoxicity evaluation both <i>in vivo</i> and <i>in vitro</i>? <i>Ecotoxicol. Environ. Saf. 169</i>: 529-538. <a href=\"https://dx.doi.org/10.1016/j.ecoenv.2018.11.066\" target=\"_blank\">https://dx.doi.org/10.1016/j.ecoenv.2018.11.066</a>","BEntID":296250,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":" <i>Ecotoxicol. Environ. Saf. 169</i>: 529-538. <a href=\"https://dx.doi.org/10.1016/j.ecoenv.2018.11.066\" target=\"_blank\">https://dx.doi.org/10.1016/j.ecoenv.2018.11.066</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Qu, M.; Ding, J.; Wang, Y.; Chen, S.; Zhang, Y.; Di, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Qu, M. <i>et al.</i>","Englishabstract":"Benzo(α)pyrene (BaP) and lead (Pb) are common pollutants discharged greatly in ocean and causing detrimental impacts on marine organisms. Although mussels are one of the most prominent and frequently studied biological models, the research on their genomic alterations induced by the mixture of two totally different chemicals, is still rare. In present study, local marine mussels Mytilus coruscus were exposed in vivo to BaP (53.74 ± 19.79 μg/ L), Pb (2.58 ± 0.11 mg/L) and their mixture for 6 days. The genotoxic damages were assessed by comet assay, micronucleus (MNi) test, and random amplified polymorphic DNA (RAPD) analysis. Significantly increased though transitory genomic damage was investigated after the exposure and showed consistency using various detecting methods. Additive genotoxicity was only found after 3 days combined exposure by means of MNi test, suggesting that BaP and Pb may play with alternative biological targets during metabolism and/or interaction with the genome. The geno-stability and the recovery capability were further detected both in vivo and in vitro after challenged by BaP. RAPD results showed coherence in BaP induced genotoxicity, together with timespecific alterations. The genomic instability was found to recover in both in vivo and in vitro exposure scenarios in present study. To our knowledge, this is the first study to focus on the genotoxicitiy induced by BaP, Pb and their mixture by multiple detecting techniques. The attempt to utilize model pollutants and marine organism to validate the potential value of RAPD analysis highlighted that it might be a useful tool in the research of genotoxicology, especially on the effect-mechanism interplay at genetic level.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Genetic impacts induced by BaP and Pb in <i>Mytilus coruscus</i>: Can RAPD be a validated tool in genotoxicity evaluation both <i>in vivo</i> and <i>in vitro</i>?","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-27 01:31:24.378413","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Multiple end-points; BaP/Pb; In vivo/vitro experiment","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2018-12-14","DateCreate":"2018-12-11","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000456226600064","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.ecoenv.2018.11.066"},"refs":null,"anarec":{"AnaID":303956,"PubliDate":2019,"Pagination":"529-538","XtraPublOfAnaID":null,"ISBN":null,"Volume":"169","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42636,"SerRR":"Ecotoxicology and Environmental Safety. Academic Press/Elsevier: Amsterdam, Netherlands etc.  ISSN 0147-6513; e-ISSN 1090-2414","StandardTitleSer":"Ecotoxicology and Environmental Safety","ISSN":"0147-6513","AbbrevSer":"Ecotoxicol. Environ. Saf.","StandardTitleMon":null,"StartPage":529,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Qu","Firstname":"Mengjie","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":296250,"AutID":355051,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Ding","Firstname":"Jiawei","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":296250,"AutID":355052,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Wang","Firstname":"Yi","Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":296250,"AutID":244086,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Chen","Firstname":"Siyu","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":296250,"AutID":355053,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Zhang","Firstname":"Yifei","Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":296250,"AutID":355054,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Di","Firstname":"Yanan","Initials":"Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":296250,"AutID":355055,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":321878,"LibID":36,"BRefID":303956,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"321878","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam, Netherlands etc"},{"PublName":"Academic Press","City":"Amsterdam, Netherlands etc"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Genotoxicity","ThestID":79950,"Acronym":"CSA","ThesTermPath":"Genotoxicity"}],"thestermsASFA":[{"ThesaurusTerm":"Genotoxicity"}],"taxtermsASFA":[{"TaxTerm":"Mytilus coruscus"}],"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"941":{"SpName":"LifeWatch Species Information Backbone","SpColID":941,"ParSpColID":39,"TopParID":39,"ShortName":"LifeWatch Species Information Backbone","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"},"507":{"SpName":"World Register of Marine Species","SpColID":507,"ParSpColID":null,"TopParID":null,"ShortName":"WoRMS website","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"WoRMS website"},"915":{"SpName":"World Register of Marine Species (WoRMS) acknowledged","SpColID":915,"ParSpColID":941,"TopParID":39,"ShortName":"WoRMS ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn"},"947":{"SpName":"WoRMS ackn - direct reference","SpColID":947,"ParSpColID":915,"TopParID":39,"ShortName":"WoRMS ackn - direct","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":39,"SpColPath":"VLIZ ackn/LifeWatch Species Information Backbone/WoRMS ackn/WoRMS ackn - direct"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":296250,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":296250,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.ecoenv.2018.11.066","externalID":"10.1016/j.ecoenv.2018.11.066","URLTypeCode":"DOI","URLID":69351,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Genotoxicity","ThestID":79950,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"}],"taxterms":[{"TaxTerm":"Mytilus coruscus","AphiaID":506159,"TaxtID":45682}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Genotoxicity","DutchTerm":null,"ThestID":79950,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"}],"taxtermsFRIS":[{"TaxTerm":"Mytilus coruscus","DutchTerm":null,"AphiaID":506159,"TaxtID":45682}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2018-12-11 13:57:43.750000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2018-12-14 09:00:21.177000","timezone_type":3,"timezone":"Europe/Brussels"}}}