{"refrec":{"BRefID":304271,"RR":"<b>Guerrero-Cruz, S.; Cremers, G.; van Alen, T.A.; Op den Camp, H.J.M.; Jetten, M.S.M.; Rasigraf, O.; Vaksmaa, A.</b> (2018). Response of the anaerobic methanotroph “<i>Candidatus</i> Methanoperedens nitroreducens” to oxygen stress. <i>Appl. Environ. Microbiol. 84(24)</i>. <a href=\"https://dx.doi.org/10.1128/aem.01832-18\" target=\"_blank\">https://dx.doi.org/10.1128/aem.01832-18</a>","BEntID":296565,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Appl. Environ. Microbiol. 84(24)</i>. <a href=\"https://dx.doi.org/10.1128/aem.01832-18\" target=\"_blank\">https://dx.doi.org/10.1128/aem.01832-18</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Guerrero-Cruz, S.; Cremers, G.; van Alen, T.A.; Op den Camp, H.J.M.; Jetten, M.S.M.; Rasigraf, O.; Vaksmaa, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Guerrero-Cruz, S. <i>et al.</i>","Englishabstract":"“<i>Candidatus</i> Methanoperedens nitroreducens” is an archaeon that couples the anaerobic oxidation of methane to nitrate reduction. In natural and man-made ecosystems, this archaeon is often found at oxic-anoxic interfaces where nitrate, the product of aerobic nitrification, cooccurs with methane produced by methanogens. As such, populations of “<i>Ca.</i> Methanoperedens nitroreducens” could be prone to regular oxygen exposure. Here, we investigated the effect of 5% (vol/vol) oxygen exposure in batch activity assays on a “<i>Ca.</i> Methanoperedens nitroreducens” culture, enriched from an Italian paddy field. Metagenome sequencing of the DNA extracted from the enrichment culture revealed that 83% of 16S rRNA gene reads were assigned to a novel strain, “<i>Candidatus</i>  Methanoperedens nitroreducens Verserenetto.” RNA was extracted, and metatranscriptome sequencing upon oxygen exposure revealed that the active community changed, most notably in the appearance of aerobic methanotrophs. The gene expression of “<i>Ca.</i>  Methanoperedens nitroreducens” revealed that the key genes encoding enzymes of the methane oxidation and nitrate reduction pathways were downregulated. In contrast to this, we identified upregulation of glutaredoxin, thioredoxin family/like proteins, rubrerythrins, peroxiredoxins, peroxidase, alkyl hydroperoxidase, type A flavoproteins, FeS cluster assembly protein, and cysteine desulfurases, indicating the genomic potential of “<i>Ca.</i>  Methanoperedens nitroreducens Verserenetto” to counteract the oxidative damage and adapt in environments where they might be exposed to regular oxygen intrusion.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Response of the anaerobic methanotroph “<i>Candidatus</i> Methanoperedens nitroreducens” to oxygen stress","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2018,"MonPub":null,"DateUpdate":"2019-01-10","DateCreate":"2018-12-20","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000451988000011","VABBcode":null,"OpenAcc":0,"DOI":"10.1128/aem.01832-18"},"refs":null,"anarec":{"AnaID":304271,"PubliDate":2018,"Pagination":null,"XtraPublOfAnaID":null,"ISBN":null,"Volume":"84","Issue":"24","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42360,"SerRR":"Applied and Environmental Microbiology. 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