{"refrec":{"BRefID":305179,"RR":"<b>Virgilio, M.; Daneel, J.-H.; Manrakhan, A.; Delatte, H.; Meganck, K.; De Meyer, M.</b> (2019). An integrated diagnostic setup for the morphological and molecular identification of the <i>Ceratitis</i> FAR complex (<i>C. anonae, C. fasciventris, C. rosa, C. quilicii</i>, Diptera, Tephritidae). <i>B. Entomol. Res. 109(3)</i>: 376-382. <a href=\"https://dx.doi.org/10.1017/s0007485318000615\" target=\"_blank\">https://dx.doi.org/10.1017/s0007485318000615</a>","BEntID":297475,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>B. Entomol. Res. 109(3)</i>: 376-382. <a href=\"https://dx.doi.org/10.1017/s0007485318000615\" target=\"_blank\">https://dx.doi.org/10.1017/s0007485318000615</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":1,"Authorstring":"Virgilio, M.; Daneel, J.-H.; Manrakhan, A.; Delatte, H.; Meganck, K.; De Meyer, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Virgilio, M. <i>et al.</i>","Englishabstract":"The <span class=\"italic\">Ceratitis</span> FAR complex (Diptera, Tephritidae) includes four economically important frugivorous flies (<span class=\"italic\">Ceratitis anonae</span>, <span class=\"italic\">Ceratitis fasciventris</span>, <span class=\"italic\">Ceratitis quilicii</span>, <span class=\"italic\">Ceratitis rosa</span>) whose immature stages and adult females cannot be properly resolved through morphological identification. In order to develop a simplified molecular tool for the identification of two of these species (<span class=\"italic\">C. rosa</span>, <span class=\"italic\">C. quilicii</span>), we selected a subset of six microsatellite markers out of a panel of 16 loci that were previously developed for the molecular differentiation of the taxa within the complex. These six markers were first tested <span class=\"italic\">in silico</span> and then used for the actual genotyping of <span class=\"italic\">C. quilicii</span> and <span class=\"italic\">C. rosa</span>, resulting in the correct identification of all male reference specimens. Here, we propose an integrated morphological and molecular setup for the identification of the four species of the FAR complex. The decision map relies on preliminary DNA barcoding or morphological identification (when possible) to exclude species not belonging to the complex followed by (a) morphological identification of all adult male specimens and female <span class=\"italic\">C. anonae</span>, (b) molecular identification via a panel of 16 microsatellite markers for immature stages, damaged vouchers and samples potentially including adult female <span class=\"italic\">C. fasciventris/C. quilicii/C. rosa</span> and (c) molecular identification via a reduced panel of six microsatellite markers for samples including only <span class=\"italic\">C. quilicii</span> and <span class=\"italic\">C. rosa</span>. This simplified diagnostic setup was profitably implemented in the framework of the ERAfrica fruit fly project and will help correctly identify species within the FAR complex for their early detection and monitoring.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"An integrated diagnostic setup for the morphological and molecular identification of the <i>Ceratitis</i> FAR complex (<i>C. anonae, C. fasciventris, C. rosa, C. quilicii</i>, Diptera, Tephritidae)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:32:45.003165","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2020-11-05","DateCreate":"2019-02-01","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000470935100011","VABBcode":null,"OpenAcc":0,"DOI":"10.1017/s0007485318000615"},"refs":null,"anarec":{"AnaID":305179,"PubliDate":2019,"Pagination":"376-382","XtraPublOfAnaID":null,"ISBN":null,"Volume":"109","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":177141,"SerRR":"Bulletin of Entomological Research. Cambridge University Press: Cambridge.  ISSN 0007-4853; e-ISSN 1475-2670","StandardTitleSer":"Bulletin of Entomological Research","ISSN":"0007-4853","AbbrevSer":"B. Entomol. 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