{"refrec":{"BRefID":307892,"RR":"<b>Rodeiro, I.; Hernandez, I.; Herrera, J.A.; Riera, M.; Donato, M.T.; Tolosa, L.; Gonzalez, K.; Ansoar, Y.; Gómez-Lechón, M.J.; Vanden Berghe, W.; Lopes, M.</b> (2018). Assessment of the cytotoxic potential of an aqueous-ethanolic extract from <i>Thalassia testudinum</i> angiosperm marine grown in the Caribbean Sea. <i>Journal of Pharmacy and Pharmacology 70(11)</i>: 1553-1560. <a href=\"https://dx.doi.org/10.1111/jphp.13001\" target=\"_blank\">https://dx.doi.org/10.1111/jphp.13001</a>","BEntID":300218,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Journal of Pharmacy and Pharmacology 70(11)</i>: 1553-1560. <a href=\"https://dx.doi.org/10.1111/jphp.13001\" target=\"_blank\">https://dx.doi.org/10.1111/jphp.13001</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Rodeiro, I.; Hernandez, I.; Herrera, J.A.; Riera, M.; Donato, M.T.; Tolosa, L.; Gonzalez, K.; Ansoar, Y.; Gómez-Lechón, M.J.; Vanden Berghe, W.; Lopes, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Rodeiro, I. <i>et al.</i>","Englishabstract":"Objectives</h3>                        <p>Reported antioxidant, anti‐inflammatory and neuroprotective properties for one aqueous‐ethanolic extract from <i>Thalassia testudinum</i> which grows in the Caribbean Sea compelled us to explore about extract cytotoxic effects.            </p>                     </div>                  <div class=\"article-section__content\" id=\"jphp13001-sec-0002\">                        <h3 class=\"article-section__sub-title section1\"> Methods</h3>                        <p>Cell viability was assayed on tumour (HepG2, PC12, Caco‐2 and 4T1) and non‐tumour (VERO, 3T3, CHO, MCDK and BHK2) cell lines. The extract effects upon primary cultures of rat and human hepatocytes and human lymphocytes were assayed.</p>                     </div>                  <div class=\"article-section__content\" id=\"jphp13001-sec-0003\">                        <h3 class=\"article-section__sub-title section1\"> Key findings</h3>                        <p>The extract exhibited cytotoxicity&nbsp;against cancer cells compared to normal cells, and the IC<sub>50</sub> values were 102&nbsp;μg/ml for HepG2, 135&nbsp;μg/ml for PC12, 165&nbsp;μg/ml for Caco‐2 and 129&nbsp;μg/ml for 4T1 cells after 48&nbsp;h, whereas IC<sub>50</sub> could not be calculated for normal cells. Additional data from a high‐content screening multiparametric assay indicated that after 24‐h exposure, the extract (up to 100&nbsp;μg/ml) induced death in HepG2 cells through oxidative stress‐associated mechanism, DNA damage and hypercalcaemia. Comet assay corroborated extract‐induced DNA damage.            </p>                     </div>                  <div class=\"article-section__content\" id=\"jphp13001-sec-0004\">                        <h3 class=\"article-section__sub-title section1\"> Conclusions</h3>                        <p><i>Thalassia testudinum</i> extract is more cytotoxic and produced more DNA damage on human hepatoma cells than to other non‐tumour cells. A possible mechanism is suggested for extract‐induced cytotoxicity based on oxidative stress, nuclear damage and hypercalcaemia in HepG2 cells. <i>T.&nbsp;testudinum</i> may be a source for antitumour agents.            </p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Assessment of the cytotoxic potential of an aqueous-ethanolic extract from <i>Thalassia testudinum</i> angiosperm marine grown in the Caribbean Sea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"cytotoxicity; DNA damage; oxidative stress; Thalassia testudinum","OtherDescriptors":null,"Notes":null,"AnaPub":2018,"MonPub":null,"DateUpdate":"2019-03-18","DateCreate":"2019-03-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000446666700011","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/jphp.13001"},"refs":null,"anarec":{"AnaID":307892,"PubliDate":2018,"Pagination":"1553-1560","XtraPublOfAnaID":null,"ISBN":null,"Volume":"70","Issue":"11","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":272930,"SerRR":"Journal of Pharmacy and Pharmacology. 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