{"refrec":{"BRefID":308049,"RR":"<b>Li, T.; Piltz, B.; Podola, B.; Dron, A.; de Beer, D.; Melkonian, M.</b> (2016). Microscale profiling of photosynthesis-related variables in a highly productive biofilm photobioreactor. <i>Biotechnol. Bioeng. 113(5)</i>: 1046-1055. <a href=\"https://dx.doi.org/10.1002/bit.25867\" target=\"_blank\">https://dx.doi.org/10.1002/bit.25867</a>","BEntID":300375,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Biotechnol. Bioeng. 113(5)</i>: 1046-1055. <a href=\"https://dx.doi.org/10.1002/bit.25867\" target=\"_blank\">https://dx.doi.org/10.1002/bit.25867</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Li, T.; Piltz, B.; Podola, B.; Dron, A.; de Beer, D.; Melkonian, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Li, T. <i>et al.</i>","Englishabstract":"In the present study depth profiles of light, oxygen, pH and photosynthetic performance in an artificial biofilm of the green alga <i>Halochlorella rubescens</i> in a porous substrate photobioreactor (PSBR) were recorded with microsensors. Biofilms were exposed to different light intensities (50–1,000 μmol photons m<sup>−2</sup> s<sup>−1</sup>) and CO<sub>2</sub> levels (0.04–5% v/v in air). The distribution of photosynthetically active radiation showed almost identical trends for different surface irradiances, namely: a relatively fast drop to a depth of about 250 µm, (to 5% of the incident), followed by a slower decrease. Light penetrated into the biofilm deeper than the Lambert‐Beer Law predicted, which may be attributed to forward scattering of light, thus improving the overall light availability. Oxygen concentration profiles showed maxima at a depth between 50 and 150 μm, depending on the incident light intensity. A very fast gas exchange was observed at the biofilm surface. The highest oxygen concentration of 3.2 mM was measured with 1,000 μmol photons m<sup>−2</sup> s<sup>−1</sup> and 5% supplementary CO<sub>2</sub>. Photosynthetic productivity increased with light intensity and/or CO<sub>2</sub> concentration and was always highest at the biofilm surface; the stimulating effect of elevated CO<sub>2</sub> concentration in the gas phase on photosynthesis was enhanced by higher light intensities. The dissolved inorganic carbon concentration profiles suggest that the availability of the dissolved free CO<sub>2</sub> has the strongest impact on photosynthetic productivity. The results suggest that dark respiration could explain previously observed decrease in growth rate over cultivation time in this type of PSBR. Our results represent a basis for understanding the complex dynamics of environmental variables and metabolic processes in artificial phototrophic biofilms exposed to a gas phase and can be used to improve the design and operational parameters of PSBRs.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Microscale profiling of photosynthesis-related variables in a highly productive biofilm photobioreactor","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:33:17.130813","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"phototrophic biofilm; microalgae; photobioreactor; microsensor;photosynthesis parameters","OtherDescriptors":null,"Notes":null,"AnaPub":2016,"MonPub":null,"DateUpdate":"2020-05-07","DateCreate":"2019-03-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000373476700013","VABBcode":null,"OpenAcc":0,"DOI":"10.1002/bit.25867"},"refs":null,"anarec":{"AnaID":308049,"PubliDate":2016,"Pagination":"1046-1055","XtraPublOfAnaID":null,"ISBN":null,"Volume":"113","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42275,"SerRR":"Biotechnology and Bioengineering. Wiley-Blackwell: New York.  ISSN 0006-3592; e-ISSN 1097-0290","StandardTitleSer":"Biotechnology and Bioengineering","ISSN":"0006-3592","AbbrevSer":"Biotechnol. 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