{"refrec":{"BRefID":310160,"RR":"<b>Peeters, E.; Nelis, H.J.; Coenye, T.</b> (2008). Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. <i>J. microbiol. methods 72(2)</i>: 157-165. <a href=\"https://dx.doi.org/10.1016/j.mimet.2007.11.010\" target=\"_blank\">https://dx.doi.org/10.1016/j.mimet.2007.11.010</a>","BEntID":302491,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>J. microbiol. methods 72(2)</i>: 157-165. <a href=\"https://dx.doi.org/10.1016/j.mimet.2007.11.010\" target=\"_blank\">https://dx.doi.org/10.1016/j.mimet.2007.11.010</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Peeters, E.; Nelis, H.J.; Coenye, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Peeters, E. <i>et al.</i>","Englishabstract":"In the present study six assays for the quantification of biofilms formed in 96-well microtiter plates were optimised and evaluated: the crystal violet (CV) assay, the Syto9 assay, the fluorescein diacetate (FDA) assay, the resazurin assay, the XTT assay and the dimethyl methylene blue (DMMB) assay. <i>Pseudomonas aeruginosa, Burkholderia cenocepacia, Staphylococcus aureus, Propionibacterium acnes</i> and <i>Candida albicans</i> were used as test organisms. In general, these assays showed a broad applicability and a high repeatability for most isolates. In addition, the estimated numbers of CFUs present in the biofilms show limited variations between the different assays. Nevertheless, our data show that some assays are less suitable for the quantification of biofilms of particular isolates (e.g. the CV assay for <i>P. aeruginosa</i>).","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-11 01:32:31.617823","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Biofilm quantification; Microtiter plate; Crystal violet; Resazurine; FDA; XTT","OtherDescriptors":null,"Notes":null,"AnaPub":2008,"MonPub":null,"DateUpdate":"2019-04-03","DateCreate":"2019-04-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000253214600007","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.mimet.2007.11.010"},"refs":null,"anarec":{"AnaID":310160,"PubliDate":2008,"Pagination":"157-165","XtraPublOfAnaID":null,"ISBN":null,"Volume":"72","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":121411,"SerRR":"Journal of Microbiological Methods. 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ISSN 0167-7012; e-ISSN 1872-8359","StandardTitleSer":"Journal of Microbiological Methods","ISSN":"0167-7012","AbbrevSer":"J. microbiol. methods","StandardTitleMon":null,"StartPage":157,"Pages":9,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Peeters","Firstname":"Elke","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":302491,"AutID":372274,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Nelis","Firstname":"Hans","Initials":"H.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":302491,"AutID":372275,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":592,"InsID":null},{"AutName":"Coenye","Firstname":"Tom","Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":302491,"AutID":242275,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0002-6407-0601","PersID":33997,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":328010,"LibID":36,"BRefID":310160,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"328010","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Elsevier","City":"Amsterdam"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":302491,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":302491,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1016/j.mimet.2007.11.010","externalID":"10.1016/j.mimet.2007.11.010","URLTypeCode":"DOI","URLID":75256,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2019-04-03 08:35:53.510000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2019-04-03 08:35:53.510000","timezone_type":3,"timezone":"Europe/Brussels"}}}