{"refrec":{"BRefID":310184,"RR":"<b>Crabbé, A.; Liu, Y.; Matthijs, N.; Rigole, P.; De La Fuente-Nùñez, C.; Davis, R.; Ledesma, M.A.; Sarker, S.; Van Houdt, R.; Hancock, R.E.W.; Coenye, T.; Nickerson, C.A.</b> (2017). Antimicrobial efficacy against <i>Pseudomonas aeruginosa</i> biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. <i>NPG Scientific Reports 7(1)</i>: 43321. <a href=\"https://dx.doi.org/10.1038/srep43321\" target=\"_blank\">https://dx.doi.org/10.1038/srep43321</a>","BEntID":302515,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 7(1)</i>: 43321. <a href=\"https://dx.doi.org/10.1038/srep43321\" target=\"_blank\">https://dx.doi.org/10.1038/srep43321</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":1,"Authorstring":"Crabbé, A.; Liu, Y.; Matthijs, N.; Rigole, P.; De La Fuente-Nùñez, C.; Davis, R.; Ledesma, M.A.; Sarker, S.; Van Houdt, R.; Hancock, R.E.W.; Coenye, T.; Nickerson, C.A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Crabbé, A. <i>et al.</i>","Englishabstract":"<i>In vitro</i> models that mimic <i>in vivo</i> host-pathogen interactions are needed to evaluate candidate drugs that inhibit bacterial virulence traits. We established a new approach to study <i>Pseudomonas aeruginosa</i> biofilm susceptibility on biotic surfaces, using a three-dimensional (3-D) lung epithelial cell model. <i>P. aeruginosa</i> formed antibiotic resistant biofilms on 3-D cells without affecting cell viability. The biofilm-inhibitory activity of antibiotics and/or the anti-biofilm peptide DJK-5 were evaluated on 3-D cells compared to a plastic surface, in medium with and without fetal bovine serum (FBS). In both media, aminoglycosides were more efficacious in the 3-D cell model. In serum-free medium, most antibiotics (except polymyxins) showed enhanced efficacy when 3-D cells were present. In medium with FBS, colistin was less efficacious in the 3-D cell model. DJK-5 exerted potent inhibition of <i>P. aeruginosa</i> association with both substrates, only in serum-free medium. DJK-5 showed stronger inhibitory activity against <i>P. aeruginosa</i> associated with plastic compared to 3-D cells. The combined addition of tobramycin and DJK-5 exhibited more potent ability to inhibit <i>P. aeruginosa</i> association with both substrates. In conclusion, lung epithelial cells influence the efficacy of most antimicrobials against <i>P. aeruginosa</i> biofilm formation, which in turn depends on the presence or absence of FBS.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Antimicrobial efficacy against <i>Pseudomonas aeruginosa</i> biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:32:09.161144","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2017,"MonPub":null,"DateUpdate":"2019-04-04","DateCreate":"2019-04-04","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000395287000002","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/srep43321"},"refs":null,"anarec":{"AnaID":310184,"PubliDate":2017,"Pagination":"43321","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). 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