{"refrec":{"BRefID":310371,"RR":"<b>Han, B.; Kaur, V.I.; Baruah, K.; Nguyen, D.V.; Bossier, P.</b> (2019). High doses of sodium ascorbate act as a prooxidant and protect gnotobiotic brine shrimp larvae (<i>Artemia franciscana</i>) against <i>Vibrio harveyi</i> infection coinciding with heat shock protein 70 activation. <i>Dev. Comp. Immunol. 92</i>: 69-76. <a href=\"https://dx.doi.org/10.1016/j.dci.2018.11.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.dci.2018.11.007</a>","BEntID":302702,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Dev. Comp. Immunol. 92</i>: 69-76. <a href=\"https://dx.doi.org/10.1016/j.dci.2018.11.007\" target=\"_blank\">https://dx.doi.org/10.1016/j.dci.2018.11.007</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Han, B.; Kaur, V.I.; Baruah, K.; Nguyen, D.V.; Bossier, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Han, B. <i>et al.</i>","Englishabstract":"Ascorbate is an essential nutrient commonly regarded as an antioxidant. In this study, using axenic brine shrimp and pathogenic strain <i>Vibrio harveyi</i> as the host-pathogen model, we confirmed that pretreatment of sodium ascorbate (NaAs), at an optimum concentration, was a prooxidant by generation of hydrogen peroxide, inducing protective effects in the brine shrimp against <i>V. harveyi</i> infection. Such a protective effect could be neutralized by the addition of an antioxidant enzyme catalase. We further showed that generation of oxygen radicals is linked to the induction of heat shock protein 70 (Hsp70), which is involved in eliciting the antioxidant protection system including superoxidase dismutase (SOD) and possibly many other immune responses. Furthermore, using RNA interferencetechnique, we found that the pretreatment of sodium ascorbate increased the survival significantly in the control knockdown groups (using green fluorescent protein, GFP) but not in Hsp70 knockdown groups and the result directly suggested that the up-regulated Hsp70 induced by sodium ascorbate pretreatment induced the protective effect. These results provide a mechanistic rationale for exploring the further use of ascorbate for antimicrobial therapy in aquaculture.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"High doses of sodium ascorbate act as a prooxidant and protect gnotobiotic brine shrimp larvae (<i>Artemia franciscana</i>) against <i>Vibrio harveyi</i> infection coinciding with heat shock protein 70 activation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:32:09.223642","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Gnotobiotic brine shrimp; Sodium ascorbate; Prooxidant; Hydrogenperoxide; Heat shock protein 70","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2019-04-24","DateCreate":"2019-04-17","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000458596200008","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.dci.2018.11.007"},"refs":null,"anarec":{"AnaID":310371,"PubliDate":2019,"Pagination":"69-76","XtraPublOfAnaID":null,"ISBN":null,"Volume":"92","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":120980,"SerRR":"Developmental and Comparative Immunology. 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