Document of bibliographic reference 312858

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

The application of DNA sequence data for the identification of benthic nematodes from the North Sea

Abstract

Nematodes or roundworms represent one of the most diverse and dominant taxon in marine benthic habitats. Whereas a morphological identification of many species is challenging, the application of molecular markers represents a promising approach for species discrimination and identification. In this study, we used an integrative taxonomic approach, combining both molecular and morphological methods, to characterize nematodes of distinct sex and ontogenetic stages from three sampling sites of the North Sea. Morphospecies were discriminated after first visual determination, followed by a molecular analysis of the nuclear 28S rDNA: D2–D3 marker. By linking each sequence to a morphological voucher, discordant morphological identification was subjected to a so-called reverse taxonomic approach. Molecular operational taxonomic units (MOTUs) and morphospecies were compared for all of the three sampling sites to assess concordance of methodology. In total, 32 MOTUs and 26 morphospecies were assigned, of which 12 taxa were identified as described species. Both approaches showed high concordance in taxon assignment (84.4 %) except for a cluster comprising various Sabatieria species. Our study revealed the high potential of the analyzed fragment as a useful molecular marker for the identification of the North Sea nematodes and highlighted the applicability of this combined taxonomic approach in general.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000344559500010

Bibliographic citation

Vogt, P.; Miljutina, M.; Raupach, M.J. (2014). The application of DNA sequence data for the identification of benthic nematodes from the North Sea. Helgol. Mar. Res. 68(4): 549-558. https://dx.doi.org/10.1007/s10152-014-0411-6

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true