{"refrec":{"BRefID":313832,"RR":"<b>Erdene-Ochir, E.; Shin, B.-K.; Kwon, B.; Jung, C.; Pan, C.-H.</b> (2019). Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from <i>Phaeodactylum tricornutum</i>. <i>NPG Scientific Reports 9(1)</i>: 10 pp. <a href=\"https://dx.doi.org/10.1038/s41598-019-45786-9\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-019-45786-9</a>","BEntID":306197,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 9(1)</i>: 10 pp. <a href=\"https://dx.doi.org/10.1038/s41598-019-45786-9\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-019-45786-9</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Erdene-Ochir, E.; Shin, B.-K.; Kwon, B.; Jung, C.; Pan, C.-H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Erdene-Ochir, E. <i>et al.</i>","Englishabstract":"Although diatoms have been extensively studied as bioreactors, only a limited number of efficient gene promoters are available. Therefore, the development of new endogenous promoters is important for the heterologous production of a variety of recombinant proteins. Herein, we identified the most abundant secreted protein in Phaeodactylum tricornutum, designated ‘highly abundant secreted protein 1’ (HASP1), and characterised the activities of its promoter and signal peptide using green fluorescent protein (GFP) as a reporter. The HASP1 promoter strongly drove GFP expression during all growth phases of <i>P. tricornutum</i> in culture, in contrast to the commonly used fcpA promoter, which is less active during the stationary phase. The HASP1 signal peptide was also sufficient for facilitating efficient secretion of GFP by P. tricornutum. Our findings suggest that both the promoter and the signal peptide of HASP1 can be utilized as novel tools for the overexpression and secretion of recombinant proteins in <i>P. tricornutum</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from <i>Phaeodactylum tricornutum</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-13 01:33:17.765187","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2019-08-08","DateCreate":"2019-08-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000474505900052","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-019-45786-9"},"refs":null,"anarec":{"AnaID":313832,"PubliDate":2019,"Pagination":"10 pp","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Erdene-Ochir","Firstname":"Erdenedolgor","Initials":"E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":306197,"AutID":388092,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Shin","Firstname":"Bok-Kyu","Initials":"B.-K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":306197,"AutID":388093,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kwon","Firstname":"Byeori","Initials":"B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":306197,"AutID":388094,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Jung","Firstname":"Choonkyun","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":306197,"AutID":388095,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Pan","Firstname":"Cheol-Ho","Initials":"C.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":306197,"AutID":388096,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":335031,"LibID":36,"BRefID":313832,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"335031","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":306197,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-019-45786-9","externalID":"10.1038/s41598-019-45786-9","URLTypeCode":"DOI","URLID":77961,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2019-08-08 09:04:06.317000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Chisala, Chilekwa, C.","updSesDate":{"date":"2019-08-08 09:04:06.317000","timezone_type":3,"timezone":"Europe/Brussels"}}}