{"refrec":{"BRefID":313959,"RR":"<b>van Losenoord, W.; Krause, J.; Parker-Nance, S.; Krause, R.; Stoychev, S.; Frost, C.L.</b> (2019). Purification and biochemical characterisation of a putative sodium channel agonist secreted from the South African Knobbly sea anemone <i>Bunodosoma capense</i>. <i>Toxicon 168</i>: 147-157. <a href=\"https://dx.doi.org/10.1016/j.toxicon.2019.06.222\" target=\"_blank\">https://dx.doi.org/10.1016/j.toxicon.2019.06.222</a>","BEntID":306324,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Toxicon 168</i>: 147-157. <a href=\"https://dx.doi.org/10.1016/j.toxicon.2019.06.222\" target=\"_blank\">https://dx.doi.org/10.1016/j.toxicon.2019.06.222</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"van Losenoord, W.; Krause, J.; Parker-Nance, S.; Krause, R.; Stoychev, S.; Frost, C.L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"van Losenoord, W. <i>et al.</i>","Englishabstract":"Voltage gated ion channels have become a subject of investigation as possible pharmaceutical targets. Research has linked the activity of ion channels directly to anti-inflammatory pathways, energy homeostasis, cancer proliferation and painful diabetic neuropathy. Sea anemones secrete a diverse array of bioactive compounds including potassium and sodium channel toxins. A putative novel sodium channel agonist (molecular mass of 4619.7 Da) with a predicted sequence: CLCNSDGPSV RGNTLSGILW LAGCPSGWHN CKKHKPTIGW CCK was isolated from <i>Bunodosoma capense</i> using a modified stimulation technique to induce the secretion of the neurotoxin rich mucus confirmed by an <i>Artemia</i> nauplii bio-assay. The peptide purification combined size-exclusion and reverse-phase high performance liquid chromatography. A thallium-based ion flux assay confirmed the presence of a sodium channel agonist/inhibitor and purity was determined using a modified tricine SDS-PAGE system. The peptide isolated indicated the presence of multiple disulfide bonds in a tight β-defensin cystine conformation. An IC50 value of 26 nM was determined for total channel inhibition on MCF-7 cells. The unique putative sodium channel agonist initiating with a cystine bond indicates a divergent evolution to those previously isolated from <i>Bunodosoma</i> species.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Purification and biochemical characterisation of a putative sodium channel agonist secreted from the South African Knobbly sea anemone <i>Bunodosoma capense</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Nav channels; Artemia bioassay; Neurotoxin; Anthozoa; ß-defensin; Sea anemone","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2019-08-14","DateCreate":"2019-08-14","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000484876000022","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.toxicon.2019.06.222"},"refs":null,"anarec":{"AnaID":313959,"PubliDate":2019,"Pagination":"147-157","XtraPublOfAnaID":null,"ISBN":null,"Volume":"168","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43961,"SerRR":"Toxicon. 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