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A series of physicochemical variables have been measured on Amsterdam Island. Part of the thesis is also a comparison of observed diatom flora with diatom flora of other sub-Antarctic islands located in Southern Indian Ocean. During the study, 76 soil samples from Amsterdam Island and 18 soil samples from Saint-Paul Island were analysed. All 193 diatom taxa were found on both islands. The results of community analysis showed that moisture, pH and conductivity are the major factors influencing the composition of diatom communities of Amsterdam Island. 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