{"refrec":{"BRefID":318137,"RR":"<b>Bale, N.J.; Palatinszky, M.; Rijpstra, W.I.C.; Herbold, C.W.; Wagner, M.; Sinninghe Damsté, J.S</b> (2019). Membrane lipid composition of the moderately thermophilic ammonia-oxidizing archaeon “<i>Candidatus</i> Nitrosotenuis uzonensis” at different growth temperatures. <i>Appl. Environ. Microbiol. 85(20)</i>. <a href=\"https://dx.doi.org/10.1128/aem.01332-19\" target=\"_blank\">https://dx.doi.org/10.1128/aem.01332-19</a>","BEntID":311574,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Appl. Environ. Microbiol. 85(20)</i>. <a href=\"https://dx.doi.org/10.1128/aem.01332-19\" target=\"_blank\">https://dx.doi.org/10.1128/aem.01332-19</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bale, N.J.; Palatinszky, M.; Rijpstra, W.I.C.; Herbold, C.W.; Wagner, M.; Sinninghe Damsté, J.S","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bale, N.J. <i>et al.</i>","Englishabstract":"“<i>Candidatus</i> Nitrosotenuis uzonensis” is the only cultured moderately thermophilic member of the thaumarchaeotal order <i>Nitrosopumilales</i> (NP) that contains many mesophilic marine strains. We examined its membrane lipid composition at different growth temperatures (37°C, 46°C, and 50°C). Its lipids were all membrane-spanning glycerol dialkyl glycerol tetraethers (GDGTs), with 0 to 4 cyclopentane moieties. Crenarchaeol (cren), the characteristic thaumarchaeotal GDGT, and its isomer (crenʹ) were present in high abundance (30 to 70%). The GDGT polar headgroups were mono-, di-, and trihexoses and hexose/phosphohexose. The ratio of glycolipid to phospholipid GDGTs was highest in the cultures grown at 50°C. With increasing growth temperatures, the relative contributions of cren and crenʹ increased, while those of GDGT-0 to GDGT-4 (including isomers) decreased. TEX<SUB>86</SUB> (tetraether index of tetraethers consisting of 86 carbons)-derived temperatures were much lower than the actual growth temperatures, further demonstrating that TEX<SUB>86</SUB> does not accurately reflect the membrane lipid adaptation of thermophilic <i>Thaumarchaeota</i>. As the temperature increased, specific GDGTs changed relative to their isomers, possibly representing temperature adaption-induced changes in cyclopentane ring stereochemistry. Comparison of a wide range of thaumarchaeotal core lipid compositions revealed that the “<i>Ca</i>. Nitrosotenuis uzonensis” cultures clustered separately from other members of the NP order and the <i>Nitrososphaerales</i> (NS) order. While phylogeny generally seems to have a strong influence on GDGT distribution, our analysis of “<i>Ca</i>. Nitrosotenuis uzonensis” demonstrates that its terrestrial, higher-temperature niche has led to a lipid composition that clearly differentiates it from other NP members and that this difference is mostly driven by its high crenʹ content.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Membrane lipid composition of the moderately thermophilic ammonia-oxidizing archaeon “<i>Candidatus</i> Nitrosotenuis uzonensis” at different growth temperatures","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"“<i>Ca</i>. 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