{"refrec":{"BRefID":318139,"RR":"<b>Bale, N.J.; Sorokin, D.Y.; Hopmans, E.C.; Koenen, M.; Rijpstra, W.I.C.; Villanueva, L.; Wienk, H.; Sinninghe Damsté, J.S.</b> (2019). New insights into the polar lipid composition of extremely halo(alkali)philic euryarchaea from hypersaline lakes. <i>Front. Microbiol. 10</i>: article 377. <a href=\"https://dx.doi.org/10.3389/fmicb.2019.00377\" target=\"_blank\">https://dx.doi.org/10.3389/fmicb.2019.00377</a>","BEntID":311576,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Front. Microbiol. 10</i>: article 377. <a href=\"https://dx.doi.org/10.3389/fmicb.2019.00377\" target=\"_blank\">https://dx.doi.org/10.3389/fmicb.2019.00377</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Bale, N.J.; Sorokin, D.Y.; Hopmans, E.C.; Koenen, M.; Rijpstra, W.I.C.; Villanueva, L.; Wienk, H.; Sinninghe Damsté, J.S.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Bale, N.J. <i>et al.</i>","Englishabstract":"We analyzed the polar membrane lipids of 13 strains of halo(alkali)philic euryarchaea from hypersaline lakes. Nine belong to the class <i>Halobacteria</i>, representing two functional groups: aerobic polysaccharide utilizers and sulfur-respiring anaerobes. The other four strains represent halo(alkali)philic methanogens from the class <i>Methanomicrobia</i> and a recently discovered class <i>Methanonatronarchaeia</i>. A wide range of polar lipids were detected across the 13 strains including dialkyl glycerol diethers (archaeols), membrane-spanning glycerol tetraethers and diether-based cardiolipins. The archaeols contained a range of core lipid structures, including combinations of C<sub>20</sub> and C<sub>25</sub> isoprenoidal alkyl chains, unsaturations, and hydroxy moieties. Several diether lipids were novel, including: (a) a phosphatidylglycerolhexose (PG-Gly) headgroup, (b) a N,N,N-trimethyl aminopentanetetrol (APT)-like lipid with a methoxy group in place of a hydroxy group on the pentanetetrol, (c) a series of polar lipids with a headgroup with elemental composition of either C<sub>12</sub>H<sub>25</sub>NO<sub>13</sub>S or C<sub>12</sub>H<sub>25</sub>NO<sub>16</sub>S<sub>2</sub>, and (d) novel cardiolipins containing a putative phosphatidylglycerolphosphate glycerophosphate (PGPGP) polar moiety. We found that the lipid distribution of the 13 strains could be generally separated into two groups, the methanogens (group) and the <i>Halobacteria</i> (class) based on the presence of specific core lipids. Within the methanogens, adaption to a high or more moderate salt concentration resulted in different ratios of glycerol dialkyl glycerol tetraethers (GDGTs) to archaeol. The methanogen <i>Methanosalsum</i>natronophilum AME2T had the most complex diether lipid composition of any of the 13 strains, including hydroxy archaeol and macrocyclic archaeol which we surmise is an order-specific membrane adaption. The zwitterionic headgroups APT and APT-Me were detected only in the <i>Methanomicrobia</i>les member <i>Methanocalculus alkaliphilus</i>AMF2T which also contained the highest level of unsaturated lipids. Only alkaliphilic members of the <i>Natrialbale</i>s order contained PGPGP cardiolipins and the PG-Gly headgroup. The four analyzed neutrophilic members of the <i>Halobacteria</i> were characterized by the presence of sulfur-containing headgroups and glycolipids. The presence of cardiolipins with one or more i-C<sub>25</sub> alkyl chains, generally termed extended archaeol (EXT-AR), in one of the <i>Methanonatronarchaeia</i> strains was unexpected as only one other order of methanogenic archaea has been reported to produce EXT-AR. We examined this further by looking into the genomic potential of various archaea to produce EXT-AR","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"New insights into the polar lipid composition of extremely halo(alkali)philic euryarchaea from hypersaline lakes","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"halo(alkali)philic; euryarchaea; haloarchaea; glycerol tetraether; membrane spanning lipids; cardiolipin; polar lipid; archaeol","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2019-11-21","DateCreate":"2019-11-21","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000460962800003","VABBcode":null,"OpenAcc":1,"DOI":"10.3389/fmicb.2019.00377"},"refs":null,"anarec":{"AnaID":318139,"PubliDate":2019,"Pagination":"article 377","XtraPublOfAnaID":null,"ISBN":null,"Volume":"10","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":234940,"SerRR":"Frontiers in Microbiology. Frontiers Media: Lausanne.  ISSN 1664-302X; e-ISSN 1664-302X","StandardTitleSer":"Frontiers in Microbiology","ISSN":"1664-302X","AbbrevSer":"Front. Microbiol.","StandardTitleMon":null,"StartPage":null,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Bale","Firstname":"Nicole","Initials":"N.J.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":211687,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":29640,"InsID":13655},{"AutName":"Sorokin","Firstname":"Dimitry","Initials":"D.Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":335739,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hopmans","Firstname":"Ellen","Initials":"E.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":210356,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-8209-1334","PersID":29774,"InsID":13655},{"AutName":"Koenen","Firstname":"Michel","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":390736,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":37981,"InsID":13655},{"AutName":"Rijpstra","Firstname":"W. Irene","Initials":"W.I.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":214855,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":29917,"InsID":13655},{"AutName":"Villanueva","Firstname":"Laura","Initials":"L.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":211317,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0001-5659-8599","PersID":29995,"InsID":13655},{"AutName":"Wienk","Firstname":"Hans","Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":396362,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Sinninghe Damsté","Firstname":"Jaap","Initials":"J.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":311576,"AutID":214921,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":"0000-0002-8683-1854","PersID":29942,"InsID":13655}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":311576,"BEntPrivate":null,"availability":[{"BInstID":340476,"LibID":2779,"BRefID":318139,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"340476","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Frontiers Media","City":"Lausanne"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":311576,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":311576,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.3389/fmicb.2019.00377","externalID":"10.3389/fmicb.2019.00377","URLTypeCode":"DOI","URLID":79663,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Leonne.van.der.Weegen@nioz.nl","newSesDate":{"date":"2019-11-21 10:06:57.530000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Leonne.van.der.Weegen@nioz.nl","updSesDate":{"date":"2019-11-21 10:06:57.530000","timezone_type":3,"timezone":"Europe/Brussels"}}}