{"refrec":{"BRefID":319598,"RR":"<b>Lee, D.-H.; Lee, Y.M.; Kim, J.-H.; Jin, Y.K.; Paull, C.; Niemann, H.; Kim, J.-H.; Shin, K.-H.</b> (2019). Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea. <i>NPG Scientific Reports 9</i>: 17592. <a href=\"https://dx.doi.org/10.1038/s41598-019-53950-4\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-019-53950-4</a>","BEntID":313037,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>NPG Scientific Reports 9</i>: 17592. <a href=\"https://dx.doi.org/10.1038/s41598-019-53950-4\" target=\"_blank\">https://dx.doi.org/10.1038/s41598-019-53950-4</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lee, D.-H.; Lee, Y.M.; Kim, J.-H.; Jin, Y.K.; Paull, C.; Niemann, H.; Kim, J.-H.; Shin, K.-H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lee, D.-H. <i>et al.</i>","Englishabstract":"Several mud volcanoes are active in the Canadian Beaufort Sea. In this study, we investigated vertical variations in methanotrophic communities in sediments of the mud volcano MV420 (420 m water depth) by analyzing geochemical properties, microbial lipids, and nucleic acid signatures. Three push cores were collected with a remotely operated vehicle from visually discriminative habitats that were devoid of megafauna and/microbial mats (DM) to the naked eye, covered with bacterial mats (BM), or colonized by siboglinid tubeworms (ST). All MV420 sites showed the presence of aerobic methane oxidation (MOx)- and anaerobic methane oxidation (AOM)-related lipid biomarkers (4α-methyl sterols and sn-2-hydroxyarchaeol, respectively), which were distinctly different in comparison with a reference site at which these compounds were not detected. Lipid biomarker results were in close agreement with 16S rRNA analyses, which revealed the presence of MOx-related bacteria (<i>Methylococcales</i>) and AOM-related archaea (ANME-2 and ANME-3) at the MV420 sites. 4α-methyl sterols derived from <i>Methylococcales</i> predominated in the surface layer at the BM site, which showed a moderate methane flux (0.04 mmol cm<sup>−2</sup> y<sup>−1</sup>), while their occurrence was limited at the DM (0.06 mmol cm<sup>−2</sup> y<sup>−1</sup>) and ST (0.01 mmol cm<sup>−2</sup> y<sup>−1</sup>) sites. On the other hand, <sup>13</sup>C-depleted <i>sn</i>-2-hydroxyarchaeol potentially derived from ANME-2 and/or ANME-3 was abundant in down-core sediments at the ST site. Our study indicates that a niche diversification within this mud volcano system has shaped distinct methanotrophic communities due to availability of electron acceptors in association with varying degrees of methane flux and bioirrigation activity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2019-12-16","DateCreate":"2019-12-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000498609100005","VABBcode":null,"OpenAcc":1,"DOI":"10.1038/s41598-019-53950-4"},"refs":null,"anarec":{"AnaID":319598,"PubliDate":2019,"Pagination":"17592","XtraPublOfAnaID":null,"ISBN":null,"Volume":"9","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":208093,"SerRR":"Scientific Reports (Nature Publishing Group). Nature Publishing Group: London.  ISSN 2045-2322; e-ISSN 2045-2322","StandardTitleSer":"Scientific Reports (Nature Publishing Group)","ISSN":"2045-2322","AbbrevSer":"NPG Scientific Reports","StandardTitleMon":null,"StartPage":17592,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lee","Firstname":"Dong-Hun","Initials":"D.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":356853,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Lee","Firstname":"Yung Mi","Initials":"Y.M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":356855,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kim","Firstname":"Jung-Hyun","Initials":"J.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":356854,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Jin","Firstname":"Young Keun","Initials":"Y.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":350274,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Paull","Firstname":"Charles","Initials":"C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":398660,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Niemann","Firstname":"Helge","Initials":"H.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":287313,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":35336,"InsID":13655},{"AutName":"Kim","Firstname":"Ji-Hoon","Initials":"J.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":246010,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Shin","Firstname":"Kyung-Hoon","Initials":"K.-H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":313037,"AutID":248324,"OrderNr":8,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":313037,"BEntPrivate":null,"availability":[{"BInstID":341126,"LibID":2779,"BRefID":319598,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"341126","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Nature Publishing Group","City":"London"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":313037,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":313037,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1038/s41598-019-53950-4","externalID":"10.1038/s41598-019-53950-4","URLTypeCode":"DOI","URLID":79991,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Leonne.van.der.Weegen@nioz.nl","newSesDate":{"date":"2019-12-16 09:29:25.363000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Leonne.van.der.Weegen@nioz.nl","updSesDate":{"date":"2019-12-16 09:29:25.363000","timezone_type":3,"timezone":"Europe/Brussels"}}}