Document of bibliographic reference 320338

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Comparison between single and multi-locus approaches for specimen identification in Mytilus mussels

Abstract

Mytilus mussels have been the object of much research given their sentinel role in coastal ecosystems and significant value as an aquaculture resource appreciated for both, its flavour and nutritional content. Some of the most-studied Mytilus species are M. edulis, M. galloprovincialis, M. chilensis and M. trossulus. As species identification based on morphological characteristics of Mytilus specimens is difficult, molecular markers are often used. Single-locus markers can give conflicting results when used independently; not all markers differentiate among all species, and the markers target genomic regions with different evolutionary histories. We evaluated the concordance between the PCR-RFLP markers most commonly-used for species identification in mussels within the Mytilus genus (Me15-16, ITS, mac-1, 16S rRNA and COI) when used alone (mono-locus approach) or together (multi-locus approach). In this study, multi-locus strategy outperformed the mono-locus methods, clearly identifying all four species and also showed similar specimen identification performance than a 49 SNPs panel. We hope that these findings will contribute to a better understanding of DNA marker-based analysis of Mytilus taxa. These results support the use of a multi-locus approach when studying this important marine resource, including research on food quality and safety, sustainable production and conservation.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000508904100023

Bibliographic citation

Larraín, M.A.; González, P.; Pérez, C.; Araneda, C. (2019). Comparison between single and multi-locus approaches for specimen identification in Mytilus mussels. NPG Scientific Reports 9(1): 19714. https://dx.doi.org/10.1038/s41598-019-55855-8

Topic

Marine

Is peer reviewed

true

Access rights

open access

Is accessible for free

true