{"refrec":{"BRefID":320602,"RR":"<b>Tahon, G.; Lebbe, L.; De Troch, M.; Sabbe, K.; Willems, A.</b> (2020). <i>Leeuwenhoekiella aestuarii</i> sp. nov., isolated from salt-water sediment and first insights in the genomes of <i>Leeuwenhoekiella</i> species. <i>Int. J. Syst. Evol. Microbiol. 70(3)</i>: 1706-1719. <a href=\"https://dx.doi.org/10.1099/ijsem.0.003959\" target=\"_blank\">https://dx.doi.org/10.1099/ijsem.0.003959</a>","BEntID":314041,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Int. J. Syst. Evol. Microbiol. 70(3)</i>: 1706-1719. <a href=\"https://dx.doi.org/10.1099/ijsem.0.003959\" target=\"_blank\">https://dx.doi.org/10.1099/ijsem.0.003959</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tahon, G.; Lebbe, L.; De Troch, M.; Sabbe, K.; Willems, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tahon, G. <i>et al.</i>","Englishabstract":"Four Gram-negative, aerobic, rod-shaped and yellow-orange pigmented bacteria (R-46770, R-48165T, R-50232 and R-50233) were isolated from intertidal sediment and water of the Westerschelde estuary between 2006 and 2012. Analysis of their 16S rRNA gene sequences revealed that the four strains form a separate cluster between validly described type strains of the genus <i>Leeuwenhoekiella</i>. DNA–DNA reassociation values of two representative strains (i.e. R-48165<sup>T</sup> and R-50232) of the new group with type strains of <i>Leeuwenhoekiella</i> species ranged from 18.7 to 56.6 %. A comparative genome analysis of the two strains and the type strains confirmed average nucleotide identity values from 75.6 to 94.4 %. The G+C contents of the genomic DNA of strains R-48165<sup>T</sup> and R-50232 were 37.80 and 37.83 mol%, respectively. The predominant cellular fatty acids of the four novel strains were summed feature 3 (i.e. C<sub>16:1</sub>ω7c and/or iso-C <sub>15:0</sub> 2-OH), iso-C <sub>15:0</sub>, iso-C <sub>15:1</sub> G and iso-C <sub>17:0</sub> 3-OH. The four new <i>Leeuwenhoekiella</i>-like strains grew with 0.5–12 % (w/v) NaCl, at pH 5.5–9.0 and displayed optimum growth between 20 and 30 °C. Based on the results of phenotypic, genomic, phylogenetic and chemotaxonomic analyses, the four new strains represent a novel species of the genus <i>Leeuwenhoekiella</i> for which the name <i>Leeuwenhoekiella aestuarii</i> sp. nov. is proposed. The type strain is LMG 30908<sup>T</sup> (=R-48165<sup>T</sup>=CECT 9775<sup>T</sup>=DSM 107866<sup>T</sup>). Genome analysis of type strains of the genus <i>Leeuwenhoekiella</i> revealed a large number of glycosyl hydrolases, peptidases and carboxyl esterases per Mb, whereas the number of transporters per Mb was low compared to other bacteria. This confirmed the environmental role of <i>Leeuwenhoekiella</i> species as (bio) polymer degraders, with a specialization on degrading proteins and high molecular weight compounds. Additionally, the presence of a large number of genes involved in gliding motility and surface adhesion, and large numbers of glycosyl transferases per Mb confirmed the importance of these features for <i>Leeuwenhoekiella</i> species.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"<i>Leeuwenhoekiella aestuarii</i> sp. nov., isolated from salt-water sediment and first insights in the genomes of <i>Leeuwenhoekiella</i> species","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-21 01:32:50.003614","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Leeuwenhoekiella, intertidal sediment and diatom ","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2021-04-07","DateCreate":"2020-01-09","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000522929700037","VABBcode":null,"OpenAcc":1,"DOI":"10.1099/ijsem.0.003959"},"refs":null,"anarec":{"AnaID":320602,"PubliDate":2020,"Pagination":"1706-1719","XtraPublOfAnaID":null,"ISBN":null,"Volume":"70","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":56795,"SerRR":"International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading.  ISSN 1466-5026; e-ISSN 1466-5034","StandardTitleSer":"International Journal of Systematic and Evolutionary Microbiology","ISSN":"1466-5026","AbbrevSer":"Int. J. Syst. Evol. Microbiol.","StandardTitleMon":null,"StartPage":1706,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Tahon","Firstname":"Guillaume","Initials":"G.","Affiliation":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":314041,"AutID":399850,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie en Microbiologie; Laboratorium voor Microbiologie","ORCID":"0000-0001-7020-4162","PersID":35766,"InsID":1605},{"AutName":"Lebbe","Firstname":"Liesbeth","Initials":"L.","Affiliation":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":314041,"AutID":399851,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie en Microbiologie; Laboratorium voor Microbiologie","ORCID":null,"PersID":31831,"InsID":1605},{"AutName":"De Troch","Firstname":"Marleen","Initials":"M.","Affiliation":"Marine Biology, Department of Biology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":314041,"AutID":176010,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MARBIOL","InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Mariene Biologie","ORCID":"0000-0002-6800-0299","PersID":223,"InsID":13},{"AutName":"Sabbe","Firstname":"Koen","Initials":"K.","Affiliation":"Protistology and Aquatic Ecology, Department of Biology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":314041,"AutID":378098,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"PAE","InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Laboratorium voor Protistologie en Aquatische Ecologie","ORCID":"0000-0001-5163-5581","PersID":21,"InsID":732},{"AutName":"Willems","Firstname":"Anne","Initials":"A.","Affiliation":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":314041,"AutID":137467,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie en Microbiologie; Laboratorium voor Microbiologie","ORCID":"0000-0002-8421-2881","PersID":10817,"InsID":1605}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":344718,"LibID":36,"BRefID":320602,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"344718","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Society for General Microbiology","City":"Reading"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Leeuwenhoekiella"}],"geotermsASFA":null,"collections":[{"Collection":"OMA - Open Marien Archief","ShortName":"OMA"},{"Collection":"ScheldeMonitor","ShortName":"scheldemonitor"},{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"222":{"SpName":"BMB - Belgische Mariene Bibliografie","SpColID":222,"ParSpColID":null,"TopParID":null,"ShortName":"BMB","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"BMB"},"991":{"SpName":"EMBRC acknowledged","SpColID":991,"ParSpColID":null,"TopParID":null,"ShortName":"EMBRC ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"EMBRC ackn"},"1019":{"SpName":"EMBRC Acknowledged A1","SpColID":1019,"ParSpColID":991,"TopParID":991,"ShortName":"EMBRC Ackn A1","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":991,"SpColPath":"EMBRC ackn/EMBRC Ackn A1"},"867":{"SpName":"IMIS themes","SpColID":867,"ParSpColID":null,"TopParID":null,"ShortName":"IMIS themes","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"IMIS themes"},"221":{"SpName":"OMA - Open Marien Archief","SpColID":221,"ParSpColID":null,"TopParID":null,"ShortName":"OMA","URLLocation":null,"LibID":36,"OpenRepoFlag":1,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"OMA"},"27":{"SpName":"ScheldeMonitor","SpColID":27,"ParSpColID":null,"TopParID":null,"ShortName":"scheldemonitor","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"scheldemonitor"},"422":{"SpName":"Section Marine Biology","SpColID":422,"ParSpColID":null,"TopParID":null,"ShortName":"MarBiol","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"MarBiol"},"641":{"SpName":"Species diversity","SpColID":641,"ParSpColID":867,"TopParID":867,"ShortName":"Diversiteit soorten","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":867,"SpColPath":"IMIS themes/Diversiteit soorten"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":314041,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":314041,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1099/ijsem.0.003959","externalID":"10.1099/ijsem.0.003959","URLTypeCode":"DOI","URLID":80287,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Leeuwenhoekiella","AphiaID":563875,"TaxtID":50512}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Leeuwenhoekiella","DutchTerm":null,"AphiaID":563875,"TaxtID":50512}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2020-01-09 09:53:28.710000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2021-04-07 06:58:44.807000","timezone_type":3,"timezone":"Europe/Brussels"}}}