{"refrec":{"BRefID":320899,"RR":"<b>van Grinsven, S.; Sinninghe Damsté, J.S; Abdala Asbun, A.; Engelmann, J.C.; Harrison, J.; Villanueva, L.</b> (2020). Methane oxidation in anoxic lake water stimulated by nitrate and sulfate addition. <i>Environ. Microbiol. 22(2)</i>: 766-782. <a href=\"https://dx.doi.org/10.1111/1462-2920.14886\" target=\"_blank\">https://dx.doi.org/10.1111/1462-2920.14886</a>","BEntID":314338,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Environ. Microbiol. 22(2)</i>: 766-782. <a href=\"https://dx.doi.org/10.1111/1462-2920.14886\" target=\"_blank\">https://dx.doi.org/10.1111/1462-2920.14886</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"van Grinsven, S.; Sinninghe Damsté, J.S; Abdala Asbun, A.; Engelmann, J.C.; Harrison, J.; Villanueva, L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"van Grinsven, S. <i>et al.</i>","Englishabstract":"Methanotrophic bacteria play a key role in limiting methane emissions from lakes. It is generally assumed that methanotrophic bacteria are mostly active at the oxic‐anoxic transition zone in stratified lakes, where they use oxygen to oxidize methane. Here, we describe a methanotroph of the genera <i>Methylobacter</i> that is performing high‐rate (up to 72 μM day−1) methane oxidation in the anoxic hypolimnion of the temperate Lacamas Lake (Washington, USA), stimulated by both nitrate and sulfate addition. Oxic and anoxic incubations both showed active methane oxidation by a <i>Methylobacter</i> species, with anoxic rates being threefold higher. In anoxic incubations, <i>Methylobacter</i> cell numbers increased almost two orders of magnitude within 3 days, suggesting that this specific <i>Methylobacter</i> species is a facultative anaerobe with a rapid response capability. Genomic analysis revealed adaptations to oxygen‐limitation as well as pathways for mixed‐acid fermentation and H2 production. The denitrification pathway was incomplete, lacking the genes <i>narG/napA</i> and <i>nosZ</i>, allowing only for methane oxidation coupled to nitrite‐reduction. Our data suggest that <i>Methylobacter</i> can be an important driver of the conversion of methane in oxygen‐limited lake systems and potentially use alternative electron acceptors or fermentation to remain active under oxygen‐depleted conditions.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Methane oxidation in anoxic lake water stimulated by nitrate and sulfate addition","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-06 01:34:08.348106","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-03-03","DateCreate":"2020-01-13","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000505043900001","VABBcode":null,"OpenAcc":1,"DOI":"10.1111/1462-2920.14886"},"refs":null,"anarec":{"AnaID":320899,"PubliDate":2020,"Pagination":"766-782","XtraPublOfAnaID":null,"ISBN":null,"Volume":"22","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":59926,"SerRR":"Environmental Microbiology. 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