{"refrec":{"BRefID":321316,"RR":"<b>van Duyl, F.C.; Lengger, S.K.; Schouten, S.; Lundälv, T.; van Oevelen, D.; Müller, C.E.</b> (2020). Dark CO<sub>2</sub> fixation into phospholipid-derived fatty acids by the cold-water coral associated sponge <i>Hymedesmia (Stylopus) coriacea</i> (Tisler Reef, NE Skagerrak). <i>Mar. Biol. Res. 16(1)</i>: 1-17. <a href=\"https://dx.doi.org/10.1080/17451000.2019.1704019\" target=\"_blank\">https://dx.doi.org/10.1080/17451000.2019.1704019</a>","BEntID":314755,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biol. Res. 16(1)</i>: 1-17. <a href=\"https://dx.doi.org/10.1080/17451000.2019.1704019\" target=\"_blank\">https://dx.doi.org/10.1080/17451000.2019.1704019</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"van Duyl, F.C.; Lengger, S.K.; Schouten, S.; Lundälv, T.; van Oevelen, D.; Müller, C.E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"van Duyl, F.C. <i>et al.</i>","Englishabstract":"Many cold-water sponges harbour microorganisms of which the role in the sponge host remains enigmatic. Here, we show a transfer of fixed inorganic carbon by sponge-associated microbes to its host, the cold-water coral encrusting sponge <i>Hymedesmia (Stylopus) coriacea</i>. Sponge were collected at approx. 100 m depth and incubated for 1.5–2.5 days with <sup>13</sup>C labelled dissolved inorganic carbon (DIC) as tracer. Total DIC fixation rates ranged from 0.03–0.11 mmol C × mmol C(sub>sponge</sub> × d−1. <sup>13</sup>C-tracer was recovered in bacterial-specific (i.e. short and branched) and sponge-specific (very long-chained) phospholipid-derived fatty acids (PLFA's), but was not incorporated into archaeal lipids. <sup>13</sup>C-incorporation in biomarkers such as C16:1w7c and C18:1w7c indicated that nitrifying and/or sulphur-oxidizing bacteria (chemoautotrophs) were likely active in the sponge. Trophic transfer of microbially-fixed carbon to the sponge host was confirmed by recovery of label in very long chain fatty acids (VLCFA's) including C26:2 and C26:3. Tracer accumulation into several VLCFA's continued after removal of <sup>13</sup>C-DIC, while tracer in most bacteria-specific PLFA's declined, indicating a transfer and elongation of bacterial-specific PLFA's to sponge-specific PLFA's. This implies that PLFA precursors released from chemo- as well as heterotrophic microbes in sponges contributed to the synthesis of VLCFA's, identifying sponge-associated bacteria as symbionts of the sponge.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Dark CO<sub>2</sub> fixation into phospholipid-derived fatty acids by the cold-water coral associated sponge <i>Hymedesmia (Stylopus) coriacea</i> (Tisler Reef, NE Skagerrak)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-19 01:32:03.282253","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-03-03","DateCreate":"2020-01-27","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000506291000001","VABBcode":null,"OpenAcc":1,"DOI":"10.1080/17451000.2019.1704019"},"refs":null,"anarec":{"AnaID":321316,"PubliDate":2020,"Pagination":"1-17","XtraPublOfAnaID":null,"ISBN":null,"Volume":"16","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":72882,"SerRR":"Marine Biology Research. Taylor & Francis: Oslo; Basingstoke.  ISSN 1745-1000; e-ISSN 1745-1019","StandardTitleSer":"Marine Biology Research","ISSN":"1745-1000","AbbrevSer":"Mar. Biol. Res.","StandardTitleMon":null,"StartPage":1,"Pages":17,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"van Duyl","Firstname":"Fleur","Initials":"F.C.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":314755,"AutID":211327,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":29713,"InsID":13655},{"AutName":"Lengger","Firstname":"Sabine","Initials":"S.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":314755,"AutID":401389,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Schouten","Firstname":"Stefan","Initials":"S.","Affiliation":"MMB","Discriminator":null,"CorporateFlag":0,"BEntID":314755,"AutID":253156,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"MMB","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Marine Microbiology and Biogeochemistry","ORCID":null,"PersID":15342,"InsID":13655},{"AutName":"Lundälv","Firstname":"Tomas","Initials":"T.","Affiliation":"Sven Loven Centre for Marine Science, University of Gothenburg","Discriminator":null,"CorporateFlag":0,"BEntID":314755,"AutID":172005,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":4823,"InsID":null},{"AutName":"van Oevelen","Firstname":"Dick","Initials":"D.","Affiliation":"EDS","Discriminator":null,"CorporateFlag":0,"BEntID":314755,"AutID":342090,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"EDS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Estuarine and Delta Systems","ORCID":"0000-0002-1740-5317","PersID":4724,"InsID":13657},{"AutName":"Müller","Firstname":"Christina","Initials":"C.E.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":314755,"AutID":401393,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"EDS","InsFSN":"Koninklijk Nederlands Instituut voor Onderzoek der Zee; Estuarine and Delta Systems","ORCID":null,"PersID":null,"InsID":13657}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":314755,"BEntPrivate":null,"availability":[{"BInstID":342759,"LibID":2779,"BRefID":321316,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"342759","RFID":null,"PaidValue":null,"Medium":"Server","Description":null,"Acronym":null,"Library":"NIOZ","DutchTerm":null,"URL":null,"ClassifID":260,"Classification":"NIOZ Open Repository","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":null,"LoggedInReq":null,"Disclaimer":"Disclaimer_NIOZ","DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":397,"FileFormID":6,"LendableFlag":null,"PublicFlag":1,"orderLib":"NIOZ","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":805,"SERpubls":[{"PublName":"Taylor & Francis","City":"Oslo; Basingstoke"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":{"805":{"SpName":"Koninklijk Nederlands Instituut voor Onderzoek der Zee","SpColID":805,"ParSpColID":null,"TopParID":null,"ShortName":"NIOZ","URLLocation":"https://www.vliz.be/imis/nioz/imis.php?refid=","LibID":2779,"OpenRepoFlag":1,"SpTypID":1,"TopParIDNotWebsite":null,"SpColPath":"NIOZ"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":314755,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":314755,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1080/17451000.2019.1704019","externalID":"10.1080/17451000.2019.1704019","URLTypeCode":"DOI","URLID":80623,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Leonne.van.der.Weegen@nioz.nl","newSesDate":{"date":"2020-01-27 12:29:15.007000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Leonne.van.der.Weegen@nioz.nl","updSesDate":{"date":"2020-03-03 09:04:32.067000","timezone_type":3,"timezone":"Europe/Brussels"}}}