Document of bibliographic reference 321365

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

Morphological and molecular study of Contracaecum larvae with a new record of Contracaecum septentrionale in Al-Sanaf marsh southern Thi-Qar Province, Iraq

Abstract

Contracaecum nematodes are the most significantgenus of the Anisakidae because of their wide range of host species involved in their life cycles, large number of their species and their health impacts. The current study aims to present the morphological andmolecular characters offourth stage larvae of Contracaecum that isolated from N. nycticorax proventriculus as free larvae in al-Sanaf marsh, southern Iraq. Larvae of Contracaecum spp. collected from proventriculus of N. nycticorax aquatic birds werestudied morphologically and their genomic DNA isolated, the ITS-1 regions of rDNA amplified by PCR technique. Sequences alignments carried out to screen nucleotide variation in ITS-1 among samples. The alignment of the sequences between Contracaecum larvae in current study with the previously reported reference gene sequences in the GenBank showed two distinct species. The first Contracaecum septentrionale recorded for the first time in Iraq and submitted to GenBank with the accession numbers MK424799.1, the second Contracaecum microcephalumsubmitted to GenBank with the accession numbers MK424795. The analysis of the ITS-1 data for Contracaecum larvae supported the distinction between species of Contracaecum larvae.

Bibliographic citation

Mohammad, Z.A.-A.; Hbaiel, M.K. (2019). Morphological and molecular study of Contracaecum larvae with a new record of Contracaecum septentrionale in Al-Sanaf marsh southern Thi-Qar Province, Iraq. Indian Journal of Public Health Research & Development 10(10): 1271-1276. https://dx.doi.org/10.5958/0976-5506.2019.03007.9

Is peer reviewed

true