{"refrec":{"BRefID":322753,"RR":"Yamamoto, S.; Bossier, P.; Yoshimatsu, T. (2020). Biochemical characterization of Rhodomonas sp. Hf-1 strain (cryptophyte) under nitrogen starvation. Aquaculture 516: 734648. https://dx.doi.org/10.1016/j.aquaculture.2019.734648","BEntID":316224,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". Aquaculture 516: 734648. https://dx.doi.org/10.1016/j.aquaculture.2019.734648","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Yamamoto, S.; Bossier, P.; Yoshimatsu, T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Yamamoto, S.; Bossier, P.; Yoshimatsu, T.","Englishabstract":"Rhodomonas sp. Hf-1 strain (cryptophyte) has been known as an excellent feed for some marine animals in aquaculture. In this study, the Hf-1 strain was cultured under different nitrogen concentrations to evaluate the effect of N-starved conditions on its growth and biochemical profile. Two nitrate concentrations of 0.9 mM (Low-N) and 3.5 mM (High-N) were supplemented to the media. The cell suspensions were sampled on day 3 and 7 and analyzed for photosynthesis pigments, protein and relative fatty acids contents. The nitrate in Low-N and High-N cultures was exhausted by day 3 and 7, respectively, and growth in Low-N culture was significantly lower than in High-N culture. Phycoerythrin (PE) content decreased in both cultures compared to chlorophyll (Chl) a and c, and a cell-color change was only observed in Low-N culture, indicating that PE was preferentially degraded compared with Chl a and c when nitrogen in the medium was limiting. The intercellular carbon and hydrogen content in the Hf-1 cells cultured in both media showed similar changes throughout the experiment, hence C/H ratios did not differ in between. On the other hand, the intercellular nitrogen content in the Hf-1 cells cultured with High-N medium increased from 5.6 to 10.3% from day 3–7. However, Low-N culture showed a decrease in nitrogen content from 8.1 to 4.1%. Consequently, C/N ratio in Low-N and High-N culture showed entirely different tendency. Similarly, the protein content in the Hf-1 cell cultured with High-N medium increased during 4 days. In contrast, Low-N culture showed a decrease by 30% in protein content. Relative fatty acids content in the Hf-1 cell was lower in Low-N culture than High-N culture through the experiment. The dominant fatty acids in Low-N culture were poly-unsaturated fatty acids (PUFA) on day 3, which accounted for 37.4% of total fatty acid. However, the relative content of PUFA reduced to 24.7% of total fatty acid by the end of the experiment, and the dominant fatty acids shifted to saturated fatty acids (SFA). These data indicate that nitrogen starvation in the medium induced a simultaneous decrease in protein and relative fatty acids contents with the cell-color change for the Hf-1 cell, suggesting the possibility that this behavior on the cell-color change can be used as a valid indicator to determine the harvest timing of Rhodomonas species visually.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Biochemical characterization of Rhodomonas sp. Hf-1 strain (cryptophyte) under nitrogen starvation","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Rhodomonas; Nutritional value; Nitrogen starvation; Phycobiliprotein;Photosynthetic pigments","OtherDescriptors":null,"Notes":null,"AnaPub":2020,"MonPub":null,"DateUpdate":"2020-04-17","DateCreate":"2020-04-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000501480700072","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquaculture.2019.734648"},"refs":null,"anarec":{"AnaID":322753,"PubliDate":2020,"Pagination":"734648","XtraPublOfAnaID":null,"ISBN":null,"Volume":"516","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo. ISSN 0044-8486; e-ISSN 1873-5622","StandardTitleSer":"Aquaculture","ISSN":"0044-8486","AbbrevSer":"Aquaculture","StandardTitleMon":null,"StartPage":8,"Pages":null,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Yamamoto","Firstname":null,"Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":316224,"AutID":412668,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":37049,"InsID":null},{"AutName":"Bossier","Firstname":null,"Initials":"P.","Affiliation":"Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioresource Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":316224,"AutID":406321,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"ARC","InsFSN":"Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center","ORCID":"0000-0002-6165-9111","PersID":125,"InsID":22},{"AutName":"Yoshimatsu","Firstname":null,"Initials":"T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":316224,"AutID":26483,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":343941,"LibID":36,"BRefID":322753,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"343941","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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