{"refrec":{"BRefID":322918,"RR":"<b>Sintsova, O.; Kalinovskii, A.; Gladkikh, I.; Zelepuga, E.; Monastyrnaya, M.; Kim, N.; Shevchenko, L.; Peigneur, S.; Tytgat, J.; Kozlovskaya, E.; Leychenko, E.</b> (2019). Magnificamide, a β-defensin-like peptide from the mucus of the sea anemone <i>Heteractis magnifica</i>, is a strong inhibitor of mammalian α-amylases. <i>Mar. Drugs 17(10)</i>: 542. <a href=\"https://dx.doi.org/10.3390/md17100542\" target=\"_blank\">https://dx.doi.org/10.3390/md17100542</a>","BEntID":316389,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Drugs 17(10)</i>: 542. <a href=\"https://dx.doi.org/10.3390/md17100542\" target=\"_blank\">https://dx.doi.org/10.3390/md17100542</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Sintsova, O.; Kalinovskii, A.; Gladkikh, I.; Zelepuga, E.; Monastyrnaya, M.; Kim, N.; Shevchenko, L.; Peigneur, S.; Tytgat, J.; Kozlovskaya, E.; Leychenko, E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Sintsova, O. <i>et al.</i>","Englishabstract":"Sea anemones’ venom is rich in peptides acting on different biological targets, mainly on cytoplasmic membranes and ion channels. These animals are also a source of pancreatic α-amylase inhibitors, which have the ability to control the glucose level in the blood and can be used for the treatment of prediabetes and type 2 diabetes mellitus. Recently we have isolated and characterized magnificamide (44 aa, 4770 Da), the major α-amylase inhibitor of the sea anemone <span class=\"html-italic\">Heteractis magnifica</span> mucus, which shares 84% sequence identity with helianthamide from <span class=\"html-italic\">Stichodactyla helianthus</span>. Herein, we report some features in the action of a recombinant analog of magnificamide. The recombinant peptide inhibits porcine pancreatic and human saliva α-amylases with Ki’s equal to 0.17 ± 0.06 nM and 7.7 ± 1.5 nM, respectively, and does not show antimicrobial or channel modulating activities. We have concluded that the main function of magnificamide is the inhibition of α-amylases; therefore, its functionally active recombinant analog is a promising agent for further studies as a potential drug candidate for the treatment of the type 2 diabetes mellitus.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Magnificamide, a β-defensin-like peptide from the mucus of the sea anemone <i>Heteractis magnifica</i>, is a strong inhibitor of mammalian α-amylases","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:28.212364","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Cnidaria; sea anemones; venom; amylase inhibitors; defensin; diabetes","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2020-04-09","DateCreate":"2020-04-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000498064500002","VABBcode":null,"OpenAcc":1,"DOI":"10.3390/md17100542"},"refs":null,"anarec":{"AnaID":322918,"PubliDate":2019,"Pagination":"542","XtraPublOfAnaID":null,"ISBN":null,"Volume":"17","Issue":"10","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":113252,"SerRR":"Marine Drugs. 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