{"refrec":{"BRefID":322969,"RR":"<b>Muthukrishnan, S.; Defoirdt, T.; Ina-Salwany, M.Y.; Yusoff, F.M.; Shariff, M.; Ismail, S.I.; Natrah, I.</b> (2019). <i>Vibrio parahaemolyticus</i> and <i>Vibrio harveyi</i> causing Acute Hepatopancreatic Necrosis Disease (AHPND) in <i>Penaeus vannamei</i> (Boone, 1931) isolated from Malaysian shrimp ponds. <i>Aquaculture 511</i>: 734227. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2019.734227\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2019.734227</a>","BEntID":316440,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquaculture 511</i>: 734227. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2019.734227\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2019.734227</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Muthukrishnan, S.; Defoirdt, T.; Ina-Salwany, M.Y.; Yusoff, F.M.; Shariff, M.; Ismail, S.I.; Natrah, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Muthukrishnan, S. <i>et al.</i>","Englishabstract":"Acute hepatopancreatic necrosis disease (AHPND) first emerged as a new shrimp disease in 2009 that heavily affected shrimp industry leading to global economic losses. The etiological agent was previously identified as <em>Vibrio parahaemolyticus</em> that carries a plasmid containing toxins (<em>PirA</em> and <em>PirB</em>). However, recent researches revealed that <em>V. parahaemolyticus</em> is not the only bacterial species capable of causing AHPND, thus this study screened on bacterial strains with AHPND toxins from <em>Penaeus vannamei</em> shrimps in Malaysia. Out of the 86 isolated total strains, 12 AHPND positive strains were arbitrarily selected and were evaluated in <em>in vivo</em> assay using <em>Artemia franciscana</em> as a model organism. All the 12 AHPND positive strains with <em>PirA</em> and <em>PirB</em> genes demonstrated significant mortalities (<em>P</em> &lt; 0.05) of <em>A. franciscana</em> compared to the negative control. The 12 AHPND positive strains were identified using molecular methods of 16S rRNA, <em>RctB</em> and <em>RpoD</em> region amplifications belonged to the <em>Harveyi</em> clade and were closely related to <em>V. parahaemolyticus</em> and <em>Vibrio harveyi</em>. Further test showed that the yellow colony <em>V. harveyi</em> strain BpShHep24 was found to be more virulent than the green colony <em>V. parahaemolyticus</em> strain BpShHep31 in shrimp <em>P. vannamei</em> challenge test. Histological examination of shrimp hepatopancreas challenged with yellow colony <em>V. harveyi</em> strain BpShHep24 showed massive sloughing of hepatopancreas tubules of epithelial cells into the lumen, haemocyte infiltrations, proximal-to-distal lesion of hepatopancreas and collapsed tubule epithelia within 24 h.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"<i>Vibrio parahaemolyticus</i> and <i>Vibrio harveyi</i> causing Acute Hepatopancreatic Necrosis Disease (AHPND) in <i>Penaeus vannamei</i> (Boone, 1931) isolated from Malaysian shrimp ponds","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:32:52.928458","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Vibriosis; Early mortality syndrome; Whiteleg shrimp","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2020-04-22","DateCreate":"2020-04-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000480233600083","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquaculture.2019.734227"},"refs":null,"anarec":{"AnaID":322969,"PubliDate":2019,"Pagination":"734227","XtraPublOfAnaID":null,"ISBN":null,"Volume":"511","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. 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