{"refrec":{"BRefID":323159,"RR":"<b>Nguyen, H.V.; Bossier, P.; Nguyen, H.T.X.; Ludeseve, C.; Garcia-Gonzalez, L.; Nevejan, N.; De Schryver, P.</b> (2019). Does <i>Ralstonia eutropha</i>, rich in poly‐β hydroxybutyrate (PHB), protect blue mussel larvae against pathogenic <i>vibrios</i>? <i>J. Fish Dis. 42(6)</i>: 777-787. <a href=\"https://dx.doi.org/10.1111/jfd.12981\" target=\"_blank\">https://dx.doi.org/10.1111/jfd.12981</a>","BEntID":316630,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":" <i>J. Fish Dis. 42(6)</i>: 777-787. <a href=\"https://dx.doi.org/10.1111/jfd.12981\" target=\"_blank\">https://dx.doi.org/10.1111/jfd.12981</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Nguyen, H.V.; Bossier, P.; Nguyen, H.T.X.; Ludeseve, C.; Garcia-Gonzalez, L.; Nevejan, N.; De Schryver, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Nguyen, H.V. <i>et al.</i>","Englishabstract":"The natural amorphous polymer poly‐β‐hydroxybutyrate (PHB‐A: lyophilized <i>Ralstonia eutropha</i> containing 75% PHB) was used as a biological agent to control bacterial pathogens of blue mussel (<i>Mytilus edulis</i>) larvae. The larvae were supplied with PHB‐A at a concentration of 1 or 10&nbsp;mg/L for 6 or 24&nbsp;hr, followed by exposure to either the rifampicin‐resistant pathogen <i>Vibrio splendidus</i> or <i>Vibrio coralliilyticus</i> at a concentration of 10<sup>5</sup> CFU/ml. Larvae pretreated 6&nbsp;hr with PHB‐A (1&nbsp;mg/L) survived a Vibrio challenge better relative to 24&nbsp;hr pretreatment. After 96&nbsp;hr of pathogen exposure, the survival of PHB‐A‐treated mussel larvae was 1.41‐ and 1.76‐fold higher than the non‐treated larvae when challenged with <i>V.&nbsp;splendidus</i> and <i>V.&nbsp;coralliilyticus</i>, respectively. Growth inhibition of the two pathogens at four concentrations of the monomer β‐HB (1, 5, 25 and 125&nbsp;mM) was tested in vitro in LB<sub>35</sub> medium, buffered at two different pH values (pH 7 and pH 8). The highest concentration of 125&nbsp;mM significantly inhibited the pathogen growth in comparison to the lower levels. The effect of β‐HB on the production of virulence factors in the tested pathogenic Vibrios revealed a variable pattern of responses.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Does <i>Ralstonia eutropha</i>, rich in poly‐β hydroxybutyrate (PHB), protect blue mussel larvae against pathogenic <i>vibrios</i>?","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-04 01:31:35.792473","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Mytilus edulis; poly-beta-hydroxybutyrate; Vibrio; virulence factors","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2020-04-16","DateCreate":"2020-04-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000467439700001","VABBcode":null,"OpenAcc":0,"DOI":"10.1111/jfd.12981"},"refs":null,"anarec":{"AnaID":323159,"PubliDate":2019,"Pagination":"777-787","XtraPublOfAnaID":null,"ISBN":null,"Volume":"42","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44476,"SerRR":"Journal of Fish Diseases. 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