{"refrec":{"BRefID":323270,"RR":"<b>Champenois, W.; Borges, A.V.</b> (2019). Determination of dimethylsulfoniopropionate and dimethylsulfoxide in <i>Posidonia oceanica</i> leaf tissue. <i>Methodsx 6</i>: 56-62. <a href=\"https://dx.doi.org/10.1016/j.mex.2018.12.014\" target=\"_blank\">https://dx.doi.org/10.1016/j.mex.2018.12.014</a>","BEntID":316742,"PublicFlag":1,"CheckedFlag":1,"wosflag":0,"vabbflag":0,"RefStringPartII":". <i>Methodsx 6</i>: 56-62. <a href=\"https://dx.doi.org/10.1016/j.mex.2018.12.014\" target=\"_blank\">https://dx.doi.org/10.1016/j.mex.2018.12.014</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Champenois, W.; Borges, A.V.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Champenois, W.; Borges, A.V.","Englishabstract":"In order to investigate the possible use of the dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) ratio as a stress indicator of <em>Posidonia oceanica</em> a method for the determination of these quantities was developed for this type of material.</p><p id=\"spar0040\"><dl class=\"list\"><dt class=\"list-label\">•</dt><dd class=\"list-description\"><p id=\"par0005\">The method relies on gas chromatography with headspace technique, instead of the purge-and-trap technique commonly used.</p></dd><dt class=\"list-label\">•</dt><dd class=\"list-description\"><p id=\"par0010\">The method allows the determination of both DMSP and DMSO on the same sample.</p></dd><dt class=\"list-label\">•</dt><dd class=\"list-description\"><p id=\"par0015\">This method allows to quantify DMSP, DMSO and DMSP:DMSO ratio for calibration curves with a coefficient of variation around 2% and a relative error around 2% and within the ranges natural variability of DMSP and DMSO in <em>P. oceanica</em> leaf tissue.</p></dd></dl></p><p>Preliminary tests showed that DMSP in <em>P. oceanica</em> leaf tissue ranged from 20 to 200 μmol g<sup>−1</sup> of fresh weight (FW) and 2 to 5 μmol g<sub>fw</sub><sup>−1</sup> for DMSO. The DMSP:DMSO ratio ranged from 2 to 40. The quantifications were conducted with different mixtures of DMSP and DMSO by measurements of DMSP and DMSO in the same sample of <em>P. oceanica</em> leaf tissue.</p>","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Determination of dimethylsulfoniopropionate and dimethylsulfoxide in <i>Posidonia oceanica</i> leaf tissue","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Dimethylsulfoniopropionate; Dimethylsulfoxide; Posidonia oceanica","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2020-04-10","DateCreate":"2020-04-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000493729600007","VABBcode":null,"OpenAcc":1,"DOI":"10.1016/j.mex.2018.12.014"},"refs":null,"anarec":{"AnaID":323270,"PubliDate":2019,"Pagination":"56-62","XtraPublOfAnaID":null,"ISBN":null,"Volume":"6","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":274688,"SerRR":"MethodsX. 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