{"refrec":{"BRefID":324010,"RR":"<b>Ramünke, S.; de Almeida Borges, F.; von Son-de Fernex, E.; von Samson-Himmelstjerna, G.; Krücken, J.</b> (2018). Molecular marker sequences of cattle <i>Cooperia</i> species identify <i>Cooperia spatulata</i> as a morphotype of <i>Cooperia punctata</i>. <i>PLoS One 13(7)</i>: e0200390. <a href=\"https://dx.doi.org/10.1371/journal.pone.0200390\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0200390</a>","BEntID":317482,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>PLoS One 13(7)</i>: e0200390. <a href=\"https://dx.doi.org/10.1371/journal.pone.0200390\" target=\"_blank\">https://dx.doi.org/10.1371/journal.pone.0200390</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ramünke, S.; de Almeida Borges, F.; von Son-de Fernex, E.; von Samson-Himmelstjerna, G.; Krücken, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ramünke, S. <i>et al.</i>","Englishabstract":"The genus <em>Cooperia</em> includes important parasites of ruminants and currently contains 34 accepted species. However, even for those species infecting livestock, there is a considerable lack of molecular information and many species are only identifiable using subtle morphological traits. The present study aimed to provide molecular data to allow diagnosis of <em>Cooperia</em> species infecting cattle. Partial sequences of two mitochondrial (cytochrome oxidase 2, 12S rRNA gene) and two nuclear genes (isotype 1 β tubulin gene including two introns, internal transcribed spacers (ITS) were obtained from morphologically identified specimens of <em>Cooperia pectinata</em>, <em>Cooperia punctata</em> and <em>Cooperia spatulata</em> as well as from larvae of pure <em>Cooperia oncophora</em> and <em>C</em>. <em>punctata</em> laboratory isolates. Pairwise identity of ITS-2 sequences was very high and it was the only region able to identify a specimen as <em>Cooperia</em> sp. However, the ITS-2 was unreliable for diagnosis at the species level. All other marker sequences could not unequivocally be allocated to the genus <em>Cooperia</em> but allowed clear species identification with the exception of the pair <em>C</em>. <em>punctata</em>/<em>C</em>. <em>spatulata</em> for which no significant differences were found for any marker sequence. Maximum-likelihood phylogenetic analyses of individual genes as well as a multi-locus analysis covering all four sequences confirmed that specimen identified as <em>C</em>. <em>spatulata</em> were randomly distributed throughout the <em>C</em>. <em>punctata</em> cluster and formed no group of their own. In contrast, the other <em>Cooperia</em> species formed clearly separated and statistically supported clusters. These data indicate that <em>C</em>. <em>spatulata</em> is most likely only a morphotype of <em>C</em>. <em>punctata</em> and the name should be considered a synonym. Combinations of nuclear and mitochondrial markers should be used to identify morphotypes or cryptic species to benefit from excellent barcoding properties of the latter but allowing proper phylogenetic analyses and controlling for lineage sorting that might occur for mitochondrial genotypes within a species.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Molecular marker sequences of cattle <i>Cooperia</i> species identify <i>Cooperia spatulata</i> as a morphotype of <i>Cooperia punctata</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2018,"MonPub":null,"DateUpdate":"2020-05-14","DateCreate":"2020-05-14","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000437809500105","VABBcode":null,"OpenAcc":1,"DOI":"10.1371/journal.pone.0200390"},"refs":null,"anarec":{"AnaID":324010,"PubliDate":2018,"Pagination":"e0200390","XtraPublOfAnaID":null,"ISBN":null,"Volume":"13","Issue":"7","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":123954,"SerRR":"PLoS One. 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